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Echinacea-induced cytosolic Ca(2+ )elevation in HEK293
BACKGROUND: With a traditional medical use for treatment of various ailments, herbal preparations of Echinacea are now popularly used to improve immune responses. One likely mode of action is that alkamides from Echinacea bind to cannabinoid type 2 (CB2) receptors and induce a transient increase in...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002894/ https://www.ncbi.nlm.nih.gov/pubmed/21092239 http://dx.doi.org/10.1186/1472-6882-10-72 |
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author | Wu, Lankun Rowe, Eric W Jeftinija, Ksenija Jeftinija, Srdija Rizshsky, Ludmila Nikolau, Basil J McKay, Jodi Kohut, Marian Wurtele, Eve Syrkin |
author_facet | Wu, Lankun Rowe, Eric W Jeftinija, Ksenija Jeftinija, Srdija Rizshsky, Ludmila Nikolau, Basil J McKay, Jodi Kohut, Marian Wurtele, Eve Syrkin |
author_sort | Wu, Lankun |
collection | PubMed |
description | BACKGROUND: With a traditional medical use for treatment of various ailments, herbal preparations of Echinacea are now popularly used to improve immune responses. One likely mode of action is that alkamides from Echinacea bind to cannabinoid type 2 (CB2) receptors and induce a transient increase in intracellular Ca(2+). Here, we show that unidentified compounds from Echinacea purpurea induce cytosolic Ca(2+ )elevation in non-immune-related cells, which lack CB2 receptors and that the Ca(2+ )elevation is not influenced by alkamides. METHODS: A non-immune human cell line, HEK293, was chosen to evaluate E. purpurea root extracts and constituents as potential regulators of intracellular Ca(2+ )levels. Changes in cytosolic Ca(2+ )levels were monitored and visualized by intracellular calcium imaging. U73122, a phospholipase C inhibitor, and 2-aminoethoxydiphenyl borate (2-APB), an antagonist of inositol-1,4,5-trisphosphate (IP(3)) receptor, were tested to determine the mechanism of this Ca(2+ )signaling pathway. E. purpurea root ethanol extracts were fractionated by preparative HPLC, screened for bioactivity on HEK293 cells and by GC-MS for potential constituent(s) responsible for this bioactivity. RESULTS: A rapid transient increase in cytosolic Ca(2+ )levels occurs when E. purpurea extracts are applied to HEK293 cells. These stimulatory effects are phospholipase C and IP(3 )receptor dependent. Echinacea-evoked responses could not be blocked by SR 144528, a specific CB2 receptor antagonist, indicating that CB2 is not involved. Ca(2+ )elevation is sustained after the Echinacea-induced Ca(2+ )release from intracellular Ca(2+ )stores; this longer-term effect is abolished by 2-APB, indicating a possible store operated calcium entry involvement. Of 28 HPLC fractions from E. purpurea root extracts, six induce cytosolic Ca(2+ )increase. Interestingly, GC-MS analysis of these fractions, as well as treatment of HEK293 cells with known individual and combined chemicals, indicates the components thought to be responsible for the major immunomodulatory bioactivity of Echinacea do not explain the observed Ca(2+ )response. Rather, lipophilic constituents of unknown structures are associated with this bioactivity. CONCLUSIONS: Our data indicate that as yet unidentified constituents from Echinacea stimulate an IP(3 )receptor and phospholipase C mediation of cytosolic Ca(2+ )levels in non-immune mammalian cells. This pathway is distinct from that induced in immune associated cells via the CB2 receptor. |
format | Text |
id | pubmed-3002894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30028942010-12-17 Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 Wu, Lankun Rowe, Eric W Jeftinija, Ksenija Jeftinija, Srdija Rizshsky, Ludmila Nikolau, Basil J McKay, Jodi Kohut, Marian Wurtele, Eve Syrkin BMC Complement Altern Med Research Article BACKGROUND: With a traditional medical use for treatment of various ailments, herbal preparations of Echinacea are now popularly used to improve immune responses. One likely mode of action is that alkamides from Echinacea bind to cannabinoid type 2 (CB2) receptors and induce a transient increase in intracellular Ca(2+). Here, we show that unidentified compounds from Echinacea purpurea induce cytosolic Ca(2+ )elevation in non-immune-related cells, which lack CB2 receptors and that the Ca(2+ )elevation is not influenced by alkamides. METHODS: A non-immune human cell line, HEK293, was chosen to evaluate E. purpurea root extracts and constituents as potential regulators of intracellular Ca(2+ )levels. Changes in cytosolic Ca(2+ )levels were monitored and visualized by intracellular calcium imaging. U73122, a phospholipase C inhibitor, and 2-aminoethoxydiphenyl borate (2-APB), an antagonist of inositol-1,4,5-trisphosphate (IP(3)) receptor, were tested to determine the mechanism of this Ca(2+ )signaling pathway. E. purpurea root ethanol extracts were fractionated by preparative HPLC, screened for bioactivity on HEK293 cells and by GC-MS for potential constituent(s) responsible for this bioactivity. RESULTS: A rapid transient increase in cytosolic Ca(2+ )levels occurs when E. purpurea extracts are applied to HEK293 cells. These stimulatory effects are phospholipase C and IP(3 )receptor dependent. Echinacea-evoked responses could not be blocked by SR 144528, a specific CB2 receptor antagonist, indicating that CB2 is not involved. Ca(2+ )elevation is sustained after the Echinacea-induced Ca(2+ )release from intracellular Ca(2+ )stores; this longer-term effect is abolished by 2-APB, indicating a possible store operated calcium entry involvement. Of 28 HPLC fractions from E. purpurea root extracts, six induce cytosolic Ca(2+ )increase. Interestingly, GC-MS analysis of these fractions, as well as treatment of HEK293 cells with known individual and combined chemicals, indicates the components thought to be responsible for the major immunomodulatory bioactivity of Echinacea do not explain the observed Ca(2+ )response. Rather, lipophilic constituents of unknown structures are associated with this bioactivity. CONCLUSIONS: Our data indicate that as yet unidentified constituents from Echinacea stimulate an IP(3 )receptor and phospholipase C mediation of cytosolic Ca(2+ )levels in non-immune mammalian cells. This pathway is distinct from that induced in immune associated cells via the CB2 receptor. BioMed Central 2010-11-23 /pmc/articles/PMC3002894/ /pubmed/21092239 http://dx.doi.org/10.1186/1472-6882-10-72 Text en Copyright ©2010 Wu et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wu, Lankun Rowe, Eric W Jeftinija, Ksenija Jeftinija, Srdija Rizshsky, Ludmila Nikolau, Basil J McKay, Jodi Kohut, Marian Wurtele, Eve Syrkin Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title | Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title_full | Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title_fullStr | Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title_full_unstemmed | Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title_short | Echinacea-induced cytosolic Ca(2+ )elevation in HEK293 |
title_sort | echinacea-induced cytosolic ca(2+ )elevation in hek293 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002894/ https://www.ncbi.nlm.nih.gov/pubmed/21092239 http://dx.doi.org/10.1186/1472-6882-10-72 |
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