Cargando…

Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)

Cotton (Gossypium spp.) is an important crop plant that is widely grown to produce both natural textile fibers and cottonseed oil. Cotton fibers, the economically more important product of the cotton plant, are seed trichomes derived from individual cells of the epidermal layer of the seed coat. It...

Descripción completa

Detalles Bibliográficos
Autores principales: Xu, Zhanyou, Yu, John Z., Cho, Jaemin, Yu, Jing, Kohel, Russell J., Percy, Richard G.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002935/
https://www.ncbi.nlm.nih.gov/pubmed/21179551
http://dx.doi.org/10.1371/journal.pone.0014351
_version_ 1782193808153247744
author Xu, Zhanyou
Yu, John Z.
Cho, Jaemin
Yu, Jing
Kohel, Russell J.
Percy, Richard G.
author_facet Xu, Zhanyou
Yu, John Z.
Cho, Jaemin
Yu, Jing
Kohel, Russell J.
Percy, Richard G.
author_sort Xu, Zhanyou
collection PubMed
description Cotton (Gossypium spp.) is an important crop plant that is widely grown to produce both natural textile fibers and cottonseed oil. Cotton fibers, the economically more important product of the cotton plant, are seed trichomes derived from individual cells of the epidermal layer of the seed coat. It has been known for a long time that large numbers of genes determine the development of cotton fiber, and more recently it has been determined that these genes are distributed across At and Dt subgenomes of tetraploid AD cottons. In the present study, the organization and evolution of the fiber development genes were investigated through the construction of an integrated genetic and physical map of fiber development genes whose functions have been verified and confirmed. A total of 535 cotton fiber development genes, including 103 fiber transcription factors, 259 fiber development genes, and 173 SSR-contained fiber ESTs, were analyzed at the subgenome level. A total of 499 fiber related contigs were selected and assembled. Together these contigs covered about 151 Mb in physical length, or about 6.7% of the tetraploid cotton genome. Among the 499 contigs, 397 were anchored onto individual chromosomes. Results from our studies on the distribution patterns of the fiber development genes and transcription factors between the At and Dt subgenomes showed that more transcription factors were from Dt subgenome than At, whereas more fiber development genes were from At subgenome than Dt. Combining our mapping results with previous reports that more fiber QTLs were mapped in Dt subgenome than At subgenome, the results suggested a new functional hypothesis for tetraploid cotton. After the merging of the two diploid Gossypium genomes, the At subgenome has provided most of the genes for fiber development, because it continues to function similar to its fiber producing diploid A genome ancestor. On the other hand, the Dt subgenome, with its non-fiber producing D genome ancestor, provides more transcription factors that regulate the expression of the fiber genes in the At subgenome. This hypothesis would explain previously published mapping results. At the same time, this integrated map of fiber development genes would provide a framework to clone individual full-length fiber genes, to elucidate the physiological mechanisms of the fiber differentiation, elongation, and maturation, and to systematically study the functional network of these genes that interact during the process of fiber development in the tetraploid cottons.
format Text
id pubmed-3002935
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30029352010-12-21 Polyploidization Altered Gene Functions in Cotton (Gossypium spp.) Xu, Zhanyou Yu, John Z. Cho, Jaemin Yu, Jing Kohel, Russell J. Percy, Richard G. PLoS One Research Article Cotton (Gossypium spp.) is an important crop plant that is widely grown to produce both natural textile fibers and cottonseed oil. Cotton fibers, the economically more important product of the cotton plant, are seed trichomes derived from individual cells of the epidermal layer of the seed coat. It has been known for a long time that large numbers of genes determine the development of cotton fiber, and more recently it has been determined that these genes are distributed across At and Dt subgenomes of tetraploid AD cottons. In the present study, the organization and evolution of the fiber development genes were investigated through the construction of an integrated genetic and physical map of fiber development genes whose functions have been verified and confirmed. A total of 535 cotton fiber development genes, including 103 fiber transcription factors, 259 fiber development genes, and 173 SSR-contained fiber ESTs, were analyzed at the subgenome level. A total of 499 fiber related contigs were selected and assembled. Together these contigs covered about 151 Mb in physical length, or about 6.7% of the tetraploid cotton genome. Among the 499 contigs, 397 were anchored onto individual chromosomes. Results from our studies on the distribution patterns of the fiber development genes and transcription factors between the At and Dt subgenomes showed that more transcription factors were from Dt subgenome than At, whereas more fiber development genes were from At subgenome than Dt. Combining our mapping results with previous reports that more fiber QTLs were mapped in Dt subgenome than At subgenome, the results suggested a new functional hypothesis for tetraploid cotton. After the merging of the two diploid Gossypium genomes, the At subgenome has provided most of the genes for fiber development, because it continues to function similar to its fiber producing diploid A genome ancestor. On the other hand, the Dt subgenome, with its non-fiber producing D genome ancestor, provides more transcription factors that regulate the expression of the fiber genes in the At subgenome. This hypothesis would explain previously published mapping results. At the same time, this integrated map of fiber development genes would provide a framework to clone individual full-length fiber genes, to elucidate the physiological mechanisms of the fiber differentiation, elongation, and maturation, and to systematically study the functional network of these genes that interact during the process of fiber development in the tetraploid cottons. Public Library of Science 2010-12-16 /pmc/articles/PMC3002935/ /pubmed/21179551 http://dx.doi.org/10.1371/journal.pone.0014351 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Xu, Zhanyou
Yu, John Z.
Cho, Jaemin
Yu, Jing
Kohel, Russell J.
Percy, Richard G.
Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title_full Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title_fullStr Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title_full_unstemmed Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title_short Polyploidization Altered Gene Functions in Cotton (Gossypium spp.)
title_sort polyploidization altered gene functions in cotton (gossypium spp.)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3002935/
https://www.ncbi.nlm.nih.gov/pubmed/21179551
http://dx.doi.org/10.1371/journal.pone.0014351
work_keys_str_mv AT xuzhanyou polyploidizationalteredgenefunctionsincottongossypiumspp
AT yujohnz polyploidizationalteredgenefunctionsincottongossypiumspp
AT chojaemin polyploidizationalteredgenefunctionsincottongossypiumspp
AT yujing polyploidizationalteredgenefunctionsincottongossypiumspp
AT kohelrussellj polyploidizationalteredgenefunctionsincottongossypiumspp
AT percyrichardg polyploidizationalteredgenefunctionsincottongossypiumspp