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Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh

Diagnosis of visceral leishmaniasis (VL) by demonstration of parasites in tissue smears obtained from bone marrow, spleen or lymph nodes is risky, painful, and difficult. The rK-39 strip test is widely used for the diagnosis of VL using blood/serum samples in endemic countries. The aim of the study...

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Autores principales: Khan, Md Gulam Musawwir, Alam, Mohammad Shafiul, Podder, Milka Patracia, Itoh, Makoto, Jamil, Kazi M, Haque, Rashidul, Wagatsuma, Yukiko
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003646/
https://www.ncbi.nlm.nih.gov/pubmed/21110875
http://dx.doi.org/10.1186/1756-3305-3-114
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author Khan, Md Gulam Musawwir
Alam, Mohammad Shafiul
Podder, Milka Patracia
Itoh, Makoto
Jamil, Kazi M
Haque, Rashidul
Wagatsuma, Yukiko
author_facet Khan, Md Gulam Musawwir
Alam, Mohammad Shafiul
Podder, Milka Patracia
Itoh, Makoto
Jamil, Kazi M
Haque, Rashidul
Wagatsuma, Yukiko
author_sort Khan, Md Gulam Musawwir
collection PubMed
description Diagnosis of visceral leishmaniasis (VL) by demonstration of parasites in tissue smears obtained from bone marrow, spleen or lymph nodes is risky, painful, and difficult. The rK-39 strip test is widely used for the diagnosis of VL using blood/serum samples in endemic countries. The aim of the study was to evaluate the rK-39 strip test using urine sample as a non-invasive means for the diagnosis of VL. The rk-39 strip test was performed using urine from 100 suspected VL cases along with 25 disease control (malarial febrile cases) and 50 healthy control (from endemic and non-endemic areas). All the VL suspected cases were positive with the rK-39 strip test using serum. The sensitivity and specificity of the rK-39 strip test using urine samples was 95% and 93.3%, respectively, compared to serum based rK-39 test. The findings suggest that the urine based rK-39 test could be a practical and efficient tool for the diagnosis of VL patients in rural areas, particularly where resources are limited.
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spelling pubmed-30036462010-12-18 Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh Khan, Md Gulam Musawwir Alam, Mohammad Shafiul Podder, Milka Patracia Itoh, Makoto Jamil, Kazi M Haque, Rashidul Wagatsuma, Yukiko Parasit Vectors Short Report Diagnosis of visceral leishmaniasis (VL) by demonstration of parasites in tissue smears obtained from bone marrow, spleen or lymph nodes is risky, painful, and difficult. The rK-39 strip test is widely used for the diagnosis of VL using blood/serum samples in endemic countries. The aim of the study was to evaluate the rK-39 strip test using urine sample as a non-invasive means for the diagnosis of VL. The rk-39 strip test was performed using urine from 100 suspected VL cases along with 25 disease control (malarial febrile cases) and 50 healthy control (from endemic and non-endemic areas). All the VL suspected cases were positive with the rK-39 strip test using serum. The sensitivity and specificity of the rK-39 strip test using urine samples was 95% and 93.3%, respectively, compared to serum based rK-39 test. The findings suggest that the urine based rK-39 test could be a practical and efficient tool for the diagnosis of VL patients in rural areas, particularly where resources are limited. BioMed Central 2010-11-26 /pmc/articles/PMC3003646/ /pubmed/21110875 http://dx.doi.org/10.1186/1756-3305-3-114 Text en Copyright ©2010 Khan et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Khan, Md Gulam Musawwir
Alam, Mohammad Shafiul
Podder, Milka Patracia
Itoh, Makoto
Jamil, Kazi M
Haque, Rashidul
Wagatsuma, Yukiko
Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title_full Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title_fullStr Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title_full_unstemmed Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title_short Evaluation of rK-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in Bangladesh
title_sort evaluation of rk-39 strip test using urine for diagnosis of visceral leishmaniasis in an endemic area in bangladesh
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003646/
https://www.ncbi.nlm.nih.gov/pubmed/21110875
http://dx.doi.org/10.1186/1756-3305-3-114
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