Cargando…

C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila

BACKGROUND: In early Drosophila embryos, the germ plasm is localized to the posterior pole region and is partitioned into the germline progenitors, known as pole cells. Germ plasm, or pole plasm, contains the polar granules which form during oogenesis and are required for germline development. Compo...

Descripción completa

Detalles Bibliográficos
Autor principal: Anne, Joël
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003691/
https://www.ncbi.nlm.nih.gov/pubmed/21179416
http://dx.doi.org/10.1371/journal.pone.0014378
_version_ 1782193894309494784
author Anne, Joël
author_facet Anne, Joël
author_sort Anne, Joël
collection PubMed
description BACKGROUND: In early Drosophila embryos, the germ plasm is localized to the posterior pole region and is partitioned into the germline progenitors, known as pole cells. Germ plasm, or pole plasm, contains the polar granules which form during oogenesis and are required for germline development. Components of these granules are also present in the perinuclear region of the nurse cells, the nuage. One such component is Tudor (Tud) which is a large protein containing multiple Tudor domains. It was previously reported that specific Tudor domains are required for germ cell formation and Tud localization. METHODOLOGY/PRINCIPAL FINDINGS: In order to better understand the function of Tud the distribution and functional activity of fragments of Tud were analyzed. These fragments were fused to GFP and the fusion proteins were synthesized during oogenesis. Non-overlapping fragments of Tud were found to be able to localize to both the nuage and pole plasm. By introducing these fragments into a tud mutant background and testing their ability to rescue the tud phenotype, I determined that the C-terminal moiety contains the functional activity of Tud. Dividing this fragment into two parts reduces its localization in pole plasm and abolishes its activity. CONCLUSIONS/SIGNIFICANCE: I conclude that the C-terminal moiety of Tud contains all the information necessary for its localization in the nuage and pole plasm and its pole cell-forming activity. The present results challenge published data and may help refining the functional features of Tud.
format Text
id pubmed-3003691
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30036912010-12-22 C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila Anne, Joël PLoS One Research Article BACKGROUND: In early Drosophila embryos, the germ plasm is localized to the posterior pole region and is partitioned into the germline progenitors, known as pole cells. Germ plasm, or pole plasm, contains the polar granules which form during oogenesis and are required for germline development. Components of these granules are also present in the perinuclear region of the nurse cells, the nuage. One such component is Tudor (Tud) which is a large protein containing multiple Tudor domains. It was previously reported that specific Tudor domains are required for germ cell formation and Tud localization. METHODOLOGY/PRINCIPAL FINDINGS: In order to better understand the function of Tud the distribution and functional activity of fragments of Tud were analyzed. These fragments were fused to GFP and the fusion proteins were synthesized during oogenesis. Non-overlapping fragments of Tud were found to be able to localize to both the nuage and pole plasm. By introducing these fragments into a tud mutant background and testing their ability to rescue the tud phenotype, I determined that the C-terminal moiety contains the functional activity of Tud. Dividing this fragment into two parts reduces its localization in pole plasm and abolishes its activity. CONCLUSIONS/SIGNIFICANCE: I conclude that the C-terminal moiety of Tud contains all the information necessary for its localization in the nuage and pole plasm and its pole cell-forming activity. The present results challenge published data and may help refining the functional features of Tud. Public Library of Science 2010-12-17 /pmc/articles/PMC3003691/ /pubmed/21179416 http://dx.doi.org/10.1371/journal.pone.0014378 Text en Joël Anne. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Anne, Joël
C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title_full C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title_fullStr C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title_full_unstemmed C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title_short C-Terminal Moiety of Tudor Contains Its In Vivo Activity in Drosophila
title_sort c-terminal moiety of tudor contains its in vivo activity in drosophila
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003691/
https://www.ncbi.nlm.nih.gov/pubmed/21179416
http://dx.doi.org/10.1371/journal.pone.0014378
work_keys_str_mv AT annejoel cterminalmoietyoftudorcontainsitsinvivoactivityindrosophila