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Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments

Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However...

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Autores principales: Liu, Juanxu, Li, Jingyu, Wang, Huinan, Fu, Zhaodi, Liu, Juan, Yu, Yixun
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003824/
https://www.ncbi.nlm.nih.gov/pubmed/20974735
http://dx.doi.org/10.1093/jxb/erq324
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author Liu, Juanxu
Li, Jingyu
Wang, Huinan
Fu, Zhaodi
Liu, Juan
Yu, Yixun
author_facet Liu, Juanxu
Li, Jingyu
Wang, Huinan
Fu, Zhaodi
Liu, Juan
Yu, Yixun
author_sort Liu, Juanxu
collection PubMed
description Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However, the characterization of ERF genes in flower senescence has not been reported. In this study, 13 ERF cDNAs were cloned from petunia. Based on the sequence characterization, these PhERFs could be classified into four of the 12 known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Expression analyses of PhERF mRNAs were performed in corollas and gynoecia of petunia flower. The 13 PhERF genes displayed differential expression patterns and levels during natural flower senescence. Exogenous ethylene accelerates the transcription of the various PhERF genes, and silver thiosulphate (STS) decreased the transcription of several PhERF genes in corollas and gynoecia. PhERF genes of group VII showed a strong association with the rise in ethylene production in both petals and gynoecia, and might be associated particularly with flower senescence in petunia. The effect of sugar, methyl jasmonate, and the plant hormones abscisic acid, salicylic acid, and 6-benzyladenine in regulating the different PhERF transcripts was investigated. Functional nuclear localization signal analyses of two PhERF proteins (PhERF2 and PhERF3) were carried out using fluorescence microscopy. These results supported a role for petunia PhERF genes in transcriptional regulation of petunia flower senescence processes.
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spelling pubmed-30038242010-12-20 Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments Liu, Juanxu Li, Jingyu Wang, Huinan Fu, Zhaodi Liu, Juan Yu, Yixun J Exp Bot Research Papers Ethylene-responsive element-binding factor (ERF) genes constitute one of the largest transcription factor gene families in plants. In Arabidopsis and rice, only a few ERF genes have been characterized so far. Flower senescence is associated with increased ethylene production in many flowers. However, the characterization of ERF genes in flower senescence has not been reported. In this study, 13 ERF cDNAs were cloned from petunia. Based on the sequence characterization, these PhERFs could be classified into four of the 12 known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Expression analyses of PhERF mRNAs were performed in corollas and gynoecia of petunia flower. The 13 PhERF genes displayed differential expression patterns and levels during natural flower senescence. Exogenous ethylene accelerates the transcription of the various PhERF genes, and silver thiosulphate (STS) decreased the transcription of several PhERF genes in corollas and gynoecia. PhERF genes of group VII showed a strong association with the rise in ethylene production in both petals and gynoecia, and might be associated particularly with flower senescence in petunia. The effect of sugar, methyl jasmonate, and the plant hormones abscisic acid, salicylic acid, and 6-benzyladenine in regulating the different PhERF transcripts was investigated. Functional nuclear localization signal analyses of two PhERF proteins (PhERF2 and PhERF3) were carried out using fluorescence microscopy. These results supported a role for petunia PhERF genes in transcriptional regulation of petunia flower senescence processes. Oxford University Press 2011-01 2010-10-25 /pmc/articles/PMC3003824/ /pubmed/20974735 http://dx.doi.org/10.1093/jxb/erq324 Text en © 2010 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Liu, Juanxu
Li, Jingyu
Wang, Huinan
Fu, Zhaodi
Liu, Juan
Yu, Yixun
Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title_full Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title_fullStr Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title_full_unstemmed Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title_short Identification and expression analysis of ERF transcription factor genes in petunia during flower senescence and in response to hormone treatments
title_sort identification and expression analysis of erf transcription factor genes in petunia during flower senescence and in response to hormone treatments
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3003824/
https://www.ncbi.nlm.nih.gov/pubmed/20974735
http://dx.doi.org/10.1093/jxb/erq324
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