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Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse

Sustained rate-coded signals encode many types of sensory modalities. Some sensory synapses possess specialized ribbon structures, which tether vesicles, to enable high-frequency signaling. However, central synapses lack these structures, yet some can maintain signaling over a wide bandwidth. To ana...

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Detalles Bibliográficos
Autores principales: Hallermann, Stefan, Fejtova, Anna, Schmidt, Hartmut, Weyhersmüller, Annika, Silver, R. Angus, Gundelfinger, Eckart D., Eilers, Jens
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004039/
https://www.ncbi.nlm.nih.gov/pubmed/21092860
http://dx.doi.org/10.1016/j.neuron.2010.10.026
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author Hallermann, Stefan
Fejtova, Anna
Schmidt, Hartmut
Weyhersmüller, Annika
Silver, R. Angus
Gundelfinger, Eckart D.
Eilers, Jens
author_facet Hallermann, Stefan
Fejtova, Anna
Schmidt, Hartmut
Weyhersmüller, Annika
Silver, R. Angus
Gundelfinger, Eckart D.
Eilers, Jens
author_sort Hallermann, Stefan
collection PubMed
description Sustained rate-coded signals encode many types of sensory modalities. Some sensory synapses possess specialized ribbon structures, which tether vesicles, to enable high-frequency signaling. However, central synapses lack these structures, yet some can maintain signaling over a wide bandwidth. To analyze the underlying molecular mechanisms, we investigated the function of the active zone core component Bassoon in cerebellar mossy fiber to granule cell synapses. We show that short-term synaptic depression is enhanced in Bassoon knockout mice during sustained high-frequency trains but basal synaptic transmission is unaffected. Fluctuation and quantal analysis as well as quantification with constrained short-term plasticity models revealed that the vesicle reloading rate was halved in the absence of Bassoon. Thus, our data show that the cytomatrix protein Bassoon speeds the reloading of vesicles to release sites at a central excitatory synapse.
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spelling pubmed-30040392011-01-24 Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse Hallermann, Stefan Fejtova, Anna Schmidt, Hartmut Weyhersmüller, Annika Silver, R. Angus Gundelfinger, Eckart D. Eilers, Jens Neuron Article Sustained rate-coded signals encode many types of sensory modalities. Some sensory synapses possess specialized ribbon structures, which tether vesicles, to enable high-frequency signaling. However, central synapses lack these structures, yet some can maintain signaling over a wide bandwidth. To analyze the underlying molecular mechanisms, we investigated the function of the active zone core component Bassoon in cerebellar mossy fiber to granule cell synapses. We show that short-term synaptic depression is enhanced in Bassoon knockout mice during sustained high-frequency trains but basal synaptic transmission is unaffected. Fluctuation and quantal analysis as well as quantification with constrained short-term plasticity models revealed that the vesicle reloading rate was halved in the absence of Bassoon. Thus, our data show that the cytomatrix protein Bassoon speeds the reloading of vesicles to release sites at a central excitatory synapse. Cell Press 2010-11-18 /pmc/articles/PMC3004039/ /pubmed/21092860 http://dx.doi.org/10.1016/j.neuron.2010.10.026 Text en © 2010 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Hallermann, Stefan
Fejtova, Anna
Schmidt, Hartmut
Weyhersmüller, Annika
Silver, R. Angus
Gundelfinger, Eckart D.
Eilers, Jens
Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title_full Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title_fullStr Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title_full_unstemmed Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title_short Bassoon Speeds Vesicle Reloading at a Central Excitatory Synapse
title_sort bassoon speeds vesicle reloading at a central excitatory synapse
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004039/
https://www.ncbi.nlm.nih.gov/pubmed/21092860
http://dx.doi.org/10.1016/j.neuron.2010.10.026
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