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Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line
PURPOSE: It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line. METHODS: We used p16 siRNA transfection...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Pediatric Society
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004487/ https://www.ncbi.nlm.nih.gov/pubmed/21189951 http://dx.doi.org/10.3345/kjp.2010.53.7.753 |
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author | Kim, Sun-Young Lee, Kyung-Yil Jeong, Dae-Chul Kim, Hak-Ki |
author_facet | Kim, Sun-Young Lee, Kyung-Yil Jeong, Dae-Chul Kim, Hak-Ki |
author_sort | Kim, Sun-Young |
collection | PubMed |
description | PURPOSE: It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line. METHODS: We used p16 siRNA transfection to construct p16-inactivated cells by using the B-cell lymphoblast cell line NC-37. We compared glucocorticoid receptor (GR) expression, apoptosis, and cell viability between control (p16+ NC-37) and p16 siRNA-transfected (p16- NC-37) cells after a single dose of dexamethasone (DX). RESULTS: In both groups, there was a significant increase in cytoplasmic GR expression, which tended to be higher for p16+ NC-37 cells than for p16- NC37 cells at all times, and the difference at 18 h was significant (P<0.05). Similar patterns of early apoptosis were observed in both groups, and late apoptosis occurred at higher levels at 18 h when the GR had already been downregulated (P<0.05). Cell viability decreased in both groups but the degree of reduction was more severe in p16+ NC-37 cells after 18 h (P<0.05). CONCLUSION: These results suggest a relationship between GR expression and cell cycle inhibition, in which the absence of p16 leads to reduced cell sensitivity to DX. |
format | Text |
id | pubmed-3004487 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | The Korean Pediatric Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-30044872010-12-28 Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line Kim, Sun-Young Lee, Kyung-Yil Jeong, Dae-Chul Kim, Hak-Ki Korean J Pediatr Original Article PURPOSE: It has been suggested that p16 has a role in glucocorticoid (GC)-related apoptosis in leukemic cells, but the exact mechanisms have yet to be clarified. We evaluated the relationship between the GC response and p16 expression in a lymphoma cell line. METHODS: We used p16 siRNA transfection to construct p16-inactivated cells by using the B-cell lymphoblast cell line NC-37. We compared glucocorticoid receptor (GR) expression, apoptosis, and cell viability between control (p16+ NC-37) and p16 siRNA-transfected (p16- NC-37) cells after a single dose of dexamethasone (DX). RESULTS: In both groups, there was a significant increase in cytoplasmic GR expression, which tended to be higher for p16+ NC-37 cells than for p16- NC37 cells at all times, and the difference at 18 h was significant (P<0.05). Similar patterns of early apoptosis were observed in both groups, and late apoptosis occurred at higher levels at 18 h when the GR had already been downregulated (P<0.05). Cell viability decreased in both groups but the degree of reduction was more severe in p16+ NC-37 cells after 18 h (P<0.05). CONCLUSION: These results suggest a relationship between GR expression and cell cycle inhibition, in which the absence of p16 leads to reduced cell sensitivity to DX. The Korean Pediatric Society 2010-07 2010-07-31 /pmc/articles/PMC3004487/ /pubmed/21189951 http://dx.doi.org/10.3345/kjp.2010.53.7.753 Text en Copyright © 2010 by The Korean Pediatric Society http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Sun-Young Lee, Kyung-Yil Jeong, Dae-Chul Kim, Hak-Ki Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title | Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title_full | Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title_fullStr | Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title_full_unstemmed | Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title_short | Effect of p16 on glucocorticoid response in a B-cell lymphoblast cell line |
title_sort | effect of p16 on glucocorticoid response in a b-cell lymphoblast cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004487/ https://www.ncbi.nlm.nih.gov/pubmed/21189951 http://dx.doi.org/10.3345/kjp.2010.53.7.753 |
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