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The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats

PURPOSE: The neuroprotective effects of erythropoietin (EPO) have been recently shown in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma, and excitotoxicity; however, limited data are available for such effects during the neonatal periods. Therefore, we inv...

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Autores principales: Kim, Moon Sun, Seo, Yoo Kyung, Park, Hye Jin, Lee, Kye Hyang, Lee, Kyung Hoon, Choi, Eun Jin, Kim, Jin Kyung, Chung, Hai Lee, Kim, Woo Taek
Formato: Texto
Lenguaje:English
Publicado: The Korean Pediatric Society 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004504/
https://www.ncbi.nlm.nih.gov/pubmed/21189961
http://dx.doi.org/10.3345/kjp.2010.53.10.898
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author Kim, Moon Sun
Seo, Yoo Kyung
Park, Hye Jin
Lee, Kye Hyang
Lee, Kyung Hoon
Choi, Eun Jin
Kim, Jin Kyung
Chung, Hai Lee
Kim, Woo Taek
author_facet Kim, Moon Sun
Seo, Yoo Kyung
Park, Hye Jin
Lee, Kye Hyang
Lee, Kyung Hoon
Choi, Eun Jin
Kim, Jin Kyung
Chung, Hai Lee
Kim, Woo Taek
author_sort Kim, Moon Sun
collection PubMed
description PURPOSE: The neuroprotective effects of erythropoietin (EPO) have been recently shown in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma, and excitotoxicity; however, limited data are available for such effects during the neonatal periods. Therefore, we investigated whether recombinant human EPO (rHuEPO) can protect against perinatal HI brain injury via an antiapoptotic mechanism. METHODS: The left carotid artery was ligated in 7-day-old Sprague-Dawley (SD) rat pups (in vivo model). The animals were divided into 6 groups: normoxia control (NC), normoxia sham-operated (NS), hypoxia only (H), hypoxia+vehicle (HV), hypoxia+rHuEPO before a hypoxic insult (HE-B), and hypoxia+rHuEPO after a hypoxic insult (HE-A). Embryonic cortical neuronal cell culture of SD rats at 18 days gestation (in vitro model) was performed. The cultured cells were divided into 5 groups: normoxia (N), hypoxia (H), and 1, 10, and 100 IU/mL rHuEPO-treated groups. RESULTS: In the in vivo model, Bcl-2 expressions in the H and HV groups were lower than those in the NC and NS groups, whereas those in the HE-A and HE-B groups were greater than those of the H and HV groups. The expressions of Bax and caspase-3 and the ratio of Bax/Bcl-2 were in contrast to those of Bcl-2. In the in vitro model, the patterns of Bcl-2, Bax, and caspase-3 expression and Bax/Bcl-2 ratio were similar to the results obtained in the in vivo model. CONCLUSION: rHuEPO exerts neuroprotective effect against perinatal HI brain injury via an antiapoptotic mechanism.
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spelling pubmed-30045042010-12-28 The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats Kim, Moon Sun Seo, Yoo Kyung Park, Hye Jin Lee, Kye Hyang Lee, Kyung Hoon Choi, Eun Jin Kim, Jin Kyung Chung, Hai Lee Kim, Woo Taek Korean J Pediatr Original Article PURPOSE: The neuroprotective effects of erythropoietin (EPO) have been recently shown in many animal models of brain injury, including hypoxic-ischemic (HI) encephalopathy, trauma, and excitotoxicity; however, limited data are available for such effects during the neonatal periods. Therefore, we investigated whether recombinant human EPO (rHuEPO) can protect against perinatal HI brain injury via an antiapoptotic mechanism. METHODS: The left carotid artery was ligated in 7-day-old Sprague-Dawley (SD) rat pups (in vivo model). The animals were divided into 6 groups: normoxia control (NC), normoxia sham-operated (NS), hypoxia only (H), hypoxia+vehicle (HV), hypoxia+rHuEPO before a hypoxic insult (HE-B), and hypoxia+rHuEPO after a hypoxic insult (HE-A). Embryonic cortical neuronal cell culture of SD rats at 18 days gestation (in vitro model) was performed. The cultured cells were divided into 5 groups: normoxia (N), hypoxia (H), and 1, 10, and 100 IU/mL rHuEPO-treated groups. RESULTS: In the in vivo model, Bcl-2 expressions in the H and HV groups were lower than those in the NC and NS groups, whereas those in the HE-A and HE-B groups were greater than those of the H and HV groups. The expressions of Bax and caspase-3 and the ratio of Bax/Bcl-2 were in contrast to those of Bcl-2. In the in vitro model, the patterns of Bcl-2, Bax, and caspase-3 expression and Bax/Bcl-2 ratio were similar to the results obtained in the in vivo model. CONCLUSION: rHuEPO exerts neuroprotective effect against perinatal HI brain injury via an antiapoptotic mechanism. The Korean Pediatric Society 2010-10 2010-10-31 /pmc/articles/PMC3004504/ /pubmed/21189961 http://dx.doi.org/10.3345/kjp.2010.53.10.898 Text en Copyright © 2010 by The Korean Pediatric Society http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kim, Moon Sun
Seo, Yoo Kyung
Park, Hye Jin
Lee, Kye Hyang
Lee, Kyung Hoon
Choi, Eun Jin
Kim, Jin Kyung
Chung, Hai Lee
Kim, Woo Taek
The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title_full The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title_fullStr The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title_full_unstemmed The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title_short The neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
title_sort neuroprotective effect of recombinant human erythropoietin via an antiapoptotic mechanism on hypoxic-ischemic brain injury in neonatal rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004504/
https://www.ncbi.nlm.nih.gov/pubmed/21189961
http://dx.doi.org/10.3345/kjp.2010.53.10.898
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