Reconstructed Ancestral Sequences Improve Pathogen Identification Using Resequencing DNA Microarrays

We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant seque...

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Detalles Bibliográficos
Autores principales: Berthet, Nicolas, Deletoile, Alexis, Passet, Virginie, Kennedy, Giulia C., Manuguerra, Jean-Claude, Cole, Stewart T., Brisse, Sylvain
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004854/
https://www.ncbi.nlm.nih.gov/pubmed/21187950
http://dx.doi.org/10.1371/journal.pone.0015243
Descripción
Sumario:We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant sequences. This improvement was attributed to the lower sequence divergence of RASs, which also expanded the sequence space covered by the microarray. Extension of this novel microarray design strategy to viruses, antimicrobial resistance elements or toxins is straightforward.

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