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Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus
BACKGROUND: Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recen...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004892/ https://www.ncbi.nlm.nih.gov/pubmed/21143842 http://dx.doi.org/10.1186/1471-2180-10-315 |
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author | Thierry, Simon Wang, Dongying Arné, Pascal Deville, Manjula De Bruin, Barbara Nieguitsila, Adélaïde Pourcel, Christine Laroucau, Karine Chermette, René Huang, Weiyi Botterel, Françoise Guillot, Jacques |
author_facet | Thierry, Simon Wang, Dongying Arné, Pascal Deville, Manjula De Bruin, Barbara Nieguitsila, Adélaïde Pourcel, Christine Laroucau, Karine Chermette, René Huang, Weiyi Botterel, Françoise Guillot, Jacques |
author_sort | Thierry, Simon |
collection | PubMed |
description | BACKGROUND: Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recently demonstrated for the avian major pathogen Chlamydophila psittaci. In the present study, we developed a similar method for another pathogen of birds: the filamentous fungus Aspergillus fumigatus. RESULTS: We selected 10 VNTR markers located on 4 different chromosomes (1, 5, 6 and 8) of A. fumigatus. These markers were tested with 57 unrelated isolates from different hosts or their environment (53 isolates from avian species in France, China or Morocco, 3 isolates from humans collected at CHU Henri Mondor hospital in France and the reference strain CBS 144.89). The Simpson index for individual markers ranged from 0.5771 to 0.8530. A combined loci index calculated with all the markers yielded an index of 0.9994. In a second step, the panel of 10 markers was used in different epidemiological situations and tested on 277 isolates, including 62 isolates from birds in Guangxi province in China, 95 isolates collected in two duck farms in France and 120 environmental isolates from a turkey hatchery in France. A database was created with the results of the present study http://minisatellites.u-psud.fr/MLVAnet/. Three major clusters of isolates were defined by using the graphing algorithm termed Minimum Spanning Tree (MST). The first cluster comprised most of the avian isolates collected in the two duck farms in France, the second cluster comprised most of the avian isolates collected in poultry farms in China and the third one comprised most of the isolates collected in the turkey hatchery in France. CONCLUSIONS: MLVA displayed excellent discriminatory power. The method showed a good reproducibility. MST analysis revealed an interesting clustering with a clear separation between isolates according to their geographic origin rather than their respective hosts. |
format | Text |
id | pubmed-3004892 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30048922011-01-10 Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus Thierry, Simon Wang, Dongying Arné, Pascal Deville, Manjula De Bruin, Barbara Nieguitsila, Adélaïde Pourcel, Christine Laroucau, Karine Chermette, René Huang, Weiyi Botterel, Françoise Guillot, Jacques BMC Microbiol Research Article BACKGROUND: Multiple-locus variable-number tandem repeat (VNTR) analysis (MLVA) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. The usefulness of MLVA was recently demonstrated for the avian major pathogen Chlamydophila psittaci. In the present study, we developed a similar method for another pathogen of birds: the filamentous fungus Aspergillus fumigatus. RESULTS: We selected 10 VNTR markers located on 4 different chromosomes (1, 5, 6 and 8) of A. fumigatus. These markers were tested with 57 unrelated isolates from different hosts or their environment (53 isolates from avian species in France, China or Morocco, 3 isolates from humans collected at CHU Henri Mondor hospital in France and the reference strain CBS 144.89). The Simpson index for individual markers ranged from 0.5771 to 0.8530. A combined loci index calculated with all the markers yielded an index of 0.9994. In a second step, the panel of 10 markers was used in different epidemiological situations and tested on 277 isolates, including 62 isolates from birds in Guangxi province in China, 95 isolates collected in two duck farms in France and 120 environmental isolates from a turkey hatchery in France. A database was created with the results of the present study http://minisatellites.u-psud.fr/MLVAnet/. Three major clusters of isolates were defined by using the graphing algorithm termed Minimum Spanning Tree (MST). The first cluster comprised most of the avian isolates collected in the two duck farms in France, the second cluster comprised most of the avian isolates collected in poultry farms in China and the third one comprised most of the isolates collected in the turkey hatchery in France. CONCLUSIONS: MLVA displayed excellent discriminatory power. The method showed a good reproducibility. MST analysis revealed an interesting clustering with a clear separation between isolates according to their geographic origin rather than their respective hosts. BioMed Central 2010-12-08 /pmc/articles/PMC3004892/ /pubmed/21143842 http://dx.doi.org/10.1186/1471-2180-10-315 Text en Copyright ©2010 Thierry et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Thierry, Simon Wang, Dongying Arné, Pascal Deville, Manjula De Bruin, Barbara Nieguitsila, Adélaïde Pourcel, Christine Laroucau, Karine Chermette, René Huang, Weiyi Botterel, Françoise Guillot, Jacques Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title | Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title_full | Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title_fullStr | Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title_full_unstemmed | Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title_short | Multiple-locus variable-number tandem repeat analysis for molecular typing of Aspergillus fumigatus |
title_sort | multiple-locus variable-number tandem repeat analysis for molecular typing of aspergillus fumigatus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004892/ https://www.ncbi.nlm.nih.gov/pubmed/21143842 http://dx.doi.org/10.1186/1471-2180-10-315 |
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