Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid

Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using...

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Detalles Bibliográficos
Autores principales: D'Castro, Laura, Wenborn, Adam, Gros, Nathalie, Joiner, Susan, Cronier, Sabrina, Collinge, John, Wadsworth, Jonathan D. F.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004958/
https://www.ncbi.nlm.nih.gov/pubmed/21187933
http://dx.doi.org/10.1371/journal.pone.0015679

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004958/
https://www.ncbi.nlm.nih.gov/pubmed/21187933
http://dx.doi.org/10.1371/journal.pone.0015679

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