Cargando…

Isolation of Proteinase K-Sensitive Prions Using Pronase E and Phosphotungstic Acid

Disease-related prion protein, PrP(Sc), is classically distinguished from its normal cellular precursor, PrP(C), by its detergent insolubility and partial resistance to proteolysis. Molecular diagnosis of prion disease typically relies upon detection of protease-resistant fragments of PrP(Sc) using...

Descripción completa

Detalles Bibliográficos
Autores principales:	D'Castro, Laura, Wenborn, Adam, Gros, Nathalie, Joiner, Susan, Cronier, Sabrina, Collinge, John, Wadsworth, Jonathan D. F.
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2010
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3004958/ https://www.ncbi.nlm.nih.gov/pubmed/21187933 http://dx.doi.org/10.1371/journal.pone.0015679

Ejemplares similares

Detection and characterization of proteinase K-sensitive disease-related prion protein with thermolysin
por: Cronier, Sabrina, et al.
Publicado: (2008)

A novel and rapid method for obtaining high titre intact prion strains from mammalian brain
por: Wenborn, Adam, et al.
Publicado: (2015)

A structural basis for prion strain diversity
por: Manka, Szymon W., et al.
Publicado: (2023)

Ex vivo mammalian prions are formed of paired double helical prion protein fibrils
por: Terry, Cassandra, et al.
Publicado: (2016)

2.7 Å cryo-EM structure of ex vivo RML prion fibrils
por: Manka, Szymon W., et al.
Publicado: (2022)

Prion strains viewed through the lens of cryo-EM
por: Manka, Szymon W., et al.
Publicado: (2022)

Structural features distinguishing infectious ex vivo mammalian prions from non-infectious fibrillar assemblies generated in vitro
por: Terry, Cassandra, et al.
Publicado: (2019)

Mechanism of Scrapie Prion Precipitation with Phosphotungstate Anions
por: Levine, Dana J., et al.
Publicado: (2014)

The origin of the prion agent of kuru: molecular and biological strain typing
por: Wadsworth, Jonathan D.F., et al.
Publicado: (2008)

Proteinase K resistant cores of prions and amyloids
por: Kushnirov, Vitaly V., et al.
Publicado: (2019)

Sex Effects in Mouse Prion Disease Incubation Time
por: Akhtar, Shaheen, et al.
Publicado: (2011)

Letter: pronase and models for the sodium conductance
Publicado: (1975)

Molecular pathology of human prion disease
por: Wadsworth, Jonathan D. F., et al.
Publicado: (2010)

Highly infectious prions are not directly neurotoxic
por: Benilova, Iryna, et al.
Publicado: (2020)

Experimental sheep BSE prions generate the vCJD phenotype when serially passaged in transgenic mice expressing human prion protein
por: Joiner, Susan, et al.
Publicado: (2018)

Absence of spontaneous disease and comparative prion susceptibility of transgenic mice expressing mutant human prion proteins
por: Asante, Emmanuel A., et al.
Publicado: (2009)

Atypical Scrapie Prions from Sheep and Lack of Disease in Transgenic Mice Overexpressing Human Prion Protein
por: Wadsworth, Jonathan D.F., et al.
Publicado: (2013)

Destruction of Sodium Conductance Inactivation in Squid Axons Perfused with Pronase
por: Armstrong, Clay M., et al.
Publicado: (1973)

ON THE PHOSPHOTUNGSTATES OF CERTAIN AMINOACIDS
por: Levene, P. A., et al.
Publicado: (1906)

Review: Contribution of transgenic models to understanding human prion disease
por: Wadsworth, J D F, et al.
Publicado: (2010)

Evaluation of a combinatorial approach to prion inactivation using an oxidizing agent, SDS, and proteinase K
por: Smith, Jodi D, et al.
Publicado: (2013)

Chemical Characterization and Pronase Susceptibility of the Na:K Pump-Associated Phosphoprotein of Human Red Blood Cells
por: Knauf, Philip A., et al.
Publicado: (1974)

Sod1 Deficiency Reduces Incubation Time in Mouse Models of Prion Disease
por: Akhtar, Shaheen, et al.
Publicado: (2013)

Modification of a voltage-gated K+ channel from sarcoplasmic reticulum by a pronase-derived specific endopeptidase
Publicado: (1979)

Transmission Properties of Human PrP 102L Prions Challenge the Relevance of Mouse Models of GSS
por: Asante, Emmanuel A., et al.
Publicado: (2015)

Evaluating the causality of novel sequence variants in the prion protein gene by example
por: Mok, Tze How, et al.
Publicado: (2018)

Release of growth hormone from ox pituitary slices after pronase treatment
por: Schofield, JG, et al.
Publicado: (1975)

Effects of premedication with Pronase for endoscopic ultrasound of the stomach: A randomized controlled trial
por: Wang, Guo-Xin, et al.
Publicado: (2016)

A Unique Case of Membranous Lupus Nephritis Identified After Pronase Digestion
por: Kannan, Lakshmi
Publicado: (2022)

Comparison of Pronase versus Manual Dechorionation of Zebrafish Embryos for Small Molecule Treatments
por: Hasegawa, Eva H., et al.
Publicado: (2023)

Central and peripheral pathology of kuru: pathological analysis of a recent case and comparison with other forms of human prion disease
por: Brandner, Sebastian, et al.
Publicado: (2008)

The First Evaluation of Proteinase K-Resistant Prion Protein (PrP(Sc)) in Korean Appendix Specimens
por: Won, Sae-Young, et al.
Publicado: (2022)

Effectiveness of Premedication with Pronase for Visualization of the Mucosa during Endoscopy: A Randomized, Controlled Trial
por: Lee, Gyu Jin, et al.
Publicado: (2012)

Additive Effect of Pronase on the Eradication Rate of First-Line Therapy for Helicobacter pylori Infection
por: Bang, Chang Seok, et al.
Publicado: (2015)

Assessing Proteinase K Resistance of Fish Prion Proteins in a Scrapie-Infected Mouse Neuroblastoma Cell Line
por: Salta, Evgenia, et al.
Publicado: (2014)

Inherited Prion Disease A117V Is Not Simply a Proteinopathy but Produces Prions Transmissible to Transgenic Mice Expressing Homologous Prion Protein
por: Asante, Emmanuel A., et al.
Publicado: (2013)

THE REACTIVITY AND STAINING OF TISSUE PROTEINS WITH PHOSPHOTUNGSTIC ACID
por: Silverman, Lloyd, et al.
Publicado: (1969)

Overexpression of mouse prion protein in transgenic mice causes a non-transmissible spongiform encephalopathy
por: Jackson, Graham S., et al.
Publicado: (2022)

Chronic wasting disease prions are not transmissible to transgenic mice overexpressing human prion protein
por: Sandberg, Malin K., et al.
Publicado: (2010)

Physical, chemical and kinetic factors affecting prion infectivity
por: Properzi, Francesca, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_2k6teaorsatsmb73tovqg1tht8