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Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution
Pulsed electric fields can enhance interstitial transport of plasmid DNA (pDNA) in solid tumors. However, the extent of enhancement is still limited. To this end, effects of cellular resistance to electric field-mediated gene delivery were investigated. The investigation used two tumor cell lines (4...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005142/ https://www.ncbi.nlm.nih.gov/pubmed/20847751 http://dx.doi.org/10.1038/cgt.2010.51 |
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author | Henshaw, Joshua Mossop, Brian Yuan, Fan |
author_facet | Henshaw, Joshua Mossop, Brian Yuan, Fan |
author_sort | Henshaw, Joshua |
collection | PubMed |
description | Pulsed electric fields can enhance interstitial transport of plasmid DNA (pDNA) in solid tumors. However, the extent of enhancement is still limited. To this end, effects of cellular resistance to electric field-mediated gene delivery were investigated. The investigation used two tumor cell lines (4T1 and B16.F10) either in suspensions or implanted in two in vivo models (dorsal skin-fold chamber (DSC) and hind leg). The volume fraction of cells was altered by pretreatment with a hyperosmotic mannitol solution (1 M). It was observed that the pretreatment reduced the volumes of 4T1 and B16.F10 cells, suspended in an agarose gel, by 50% and 46%, respectively, over a 20-min period but did not cause significant changes ex vivo in volumes of hind leg tumor tissues grown from the same cells in mice. The mannitol pretreatment in vivo improved electric field-mediated gene delivery in the hind leg tumor models, in terms of reporter gene expression, but resulted in minimal enhancement in pDNA electrophoresis over a few micron distance in the DSC tumor models. These data demonstrated that hyperosmotic mannitol solution could effectively improve electric field-mediated gene delivery around individual cells in vivo through increasing the extracellular space. |
format | Text |
id | pubmed-3005142 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
record_format | MEDLINE/PubMed |
spelling | pubmed-30051422011-07-01 Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution Henshaw, Joshua Mossop, Brian Yuan, Fan Cancer Gene Ther Article Pulsed electric fields can enhance interstitial transport of plasmid DNA (pDNA) in solid tumors. However, the extent of enhancement is still limited. To this end, effects of cellular resistance to electric field-mediated gene delivery were investigated. The investigation used two tumor cell lines (4T1 and B16.F10) either in suspensions or implanted in two in vivo models (dorsal skin-fold chamber (DSC) and hind leg). The volume fraction of cells was altered by pretreatment with a hyperosmotic mannitol solution (1 M). It was observed that the pretreatment reduced the volumes of 4T1 and B16.F10 cells, suspended in an agarose gel, by 50% and 46%, respectively, over a 20-min period but did not cause significant changes ex vivo in volumes of hind leg tumor tissues grown from the same cells in mice. The mannitol pretreatment in vivo improved electric field-mediated gene delivery in the hind leg tumor models, in terms of reporter gene expression, but resulted in minimal enhancement in pDNA electrophoresis over a few micron distance in the DSC tumor models. These data demonstrated that hyperosmotic mannitol solution could effectively improve electric field-mediated gene delivery around individual cells in vivo through increasing the extracellular space. 2010-09-17 2011-01 /pmc/articles/PMC3005142/ /pubmed/20847751 http://dx.doi.org/10.1038/cgt.2010.51 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Henshaw, Joshua Mossop, Brian Yuan, Fan Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title | Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title_full | Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title_fullStr | Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title_full_unstemmed | Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title_short | Enhancement of Electric Field-Mediated Gene Delivery Through Pretreatment of Tumors With a Hyperosmotic Mannitol Solution |
title_sort | enhancement of electric field-mediated gene delivery through pretreatment of tumors with a hyperosmotic mannitol solution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3005142/ https://www.ncbi.nlm.nih.gov/pubmed/20847751 http://dx.doi.org/10.1038/cgt.2010.51 |
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