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TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells

Flagellum-mediated motility of Trypanosoma brucei is considered to be essential for the parasite to complete stage development in the tsetse fly vector, while the mechanism by which flagellum-mediated motility is controlled are not fully understood. We thus compared T. brucei whole gene products (am...

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Autores principales: Ohshima, Shigeru, Ohashi-Suzuki, Mitsuko, Miura, Yutaka, Yabu, Yoshisada, Okada, Noriko, Ohta, Nobuo, Suzuki, Takashi
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008729/
https://www.ncbi.nlm.nih.gov/pubmed/21203515
http://dx.doi.org/10.1371/journal.pone.0015577
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author Ohshima, Shigeru
Ohashi-Suzuki, Mitsuko
Miura, Yutaka
Yabu, Yoshisada
Okada, Noriko
Ohta, Nobuo
Suzuki, Takashi
author_facet Ohshima, Shigeru
Ohashi-Suzuki, Mitsuko
Miura, Yutaka
Yabu, Yoshisada
Okada, Noriko
Ohta, Nobuo
Suzuki, Takashi
author_sort Ohshima, Shigeru
collection PubMed
description Flagellum-mediated motility of Trypanosoma brucei is considered to be essential for the parasite to complete stage development in the tsetse fly vector, while the mechanism by which flagellum-mediated motility is controlled are not fully understood. We thus compared T. brucei whole gene products (amino acid sequence) with Caenorhabditis elegans UNC (uncoordinated) proteins, in order to find uncharacterized motility-related T. brucei genes. Through in silico analysis, we found 88 gene products which were highly similar to C. elegans UNC proteins and categorized them as TbCEUN (T. brucei gene products which have high similarity to C. elegans UNC proteins). Approximately two thirds of the 88 TbCEUN gene products were kinesin-related molecules. A gene product highly similar to C. elegans UNC119 protein was designated as TbUNC119. RNAi-mediated depletion of TbUNC119 showed no apparent phenotype. However, knock-down analysis of both TbUNC119 and its binding protein (TbUNC119BP) which was found by yeast two-hybrid analysis showed characteristic phenotypes, including reduced motility, morphological change (extended cell shape), and cellular apoptosis. Based on the observed phenotypes, possible function of the TbUNC119 and TbUNC119BP is discussed.
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spelling pubmed-30087292011-01-03 TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells Ohshima, Shigeru Ohashi-Suzuki, Mitsuko Miura, Yutaka Yabu, Yoshisada Okada, Noriko Ohta, Nobuo Suzuki, Takashi PLoS One Research Article Flagellum-mediated motility of Trypanosoma brucei is considered to be essential for the parasite to complete stage development in the tsetse fly vector, while the mechanism by which flagellum-mediated motility is controlled are not fully understood. We thus compared T. brucei whole gene products (amino acid sequence) with Caenorhabditis elegans UNC (uncoordinated) proteins, in order to find uncharacterized motility-related T. brucei genes. Through in silico analysis, we found 88 gene products which were highly similar to C. elegans UNC proteins and categorized them as TbCEUN (T. brucei gene products which have high similarity to C. elegans UNC proteins). Approximately two thirds of the 88 TbCEUN gene products were kinesin-related molecules. A gene product highly similar to C. elegans UNC119 protein was designated as TbUNC119. RNAi-mediated depletion of TbUNC119 showed no apparent phenotype. However, knock-down analysis of both TbUNC119 and its binding protein (TbUNC119BP) which was found by yeast two-hybrid analysis showed characteristic phenotypes, including reduced motility, morphological change (extended cell shape), and cellular apoptosis. Based on the observed phenotypes, possible function of the TbUNC119 and TbUNC119BP is discussed. Public Library of Science 2010-12-22 /pmc/articles/PMC3008729/ /pubmed/21203515 http://dx.doi.org/10.1371/journal.pone.0015577 Text en Ohshima et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ohshima, Shigeru
Ohashi-Suzuki, Mitsuko
Miura, Yutaka
Yabu, Yoshisada
Okada, Noriko
Ohta, Nobuo
Suzuki, Takashi
TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title_full TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title_fullStr TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title_full_unstemmed TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title_short TbUNC119 and Its Binding Protein Complex Are Essential for Propagation, Motility, and Morphogenesis of Trypanosoma brucei Procyclic Form Cells
title_sort tbunc119 and its binding protein complex are essential for propagation, motility, and morphogenesis of trypanosoma brucei procyclic form cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008729/
https://www.ncbi.nlm.nih.gov/pubmed/21203515
http://dx.doi.org/10.1371/journal.pone.0015577
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