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A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy

Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microsco...

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Detalles Bibliográficos
Autores principales: Coppieters, Ken, Martinic, Marianne M., Kiosses, William B., Amirian, Natalie, von Herrath, Matthias
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3009738/
https://www.ncbi.nlm.nih.gov/pubmed/21203413
http://dx.doi.org/10.1371/journal.pone.0015732
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author Coppieters, Ken
Martinic, Marianne M.
Kiosses, William B.
Amirian, Natalie
von Herrath, Matthias
author_facet Coppieters, Ken
Martinic, Marianne M.
Kiosses, William B.
Amirian, Natalie
von Herrath, Matthias
author_sort Coppieters, Ken
collection PubMed
description Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction.
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spelling pubmed-30097382011-01-03 A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy Coppieters, Ken Martinic, Marianne M. Kiosses, William B. Amirian, Natalie von Herrath, Matthias PLoS One Research Article Type 1 diabetes (T1D) is characterized by the immune-mediated destruction of beta cells in the pancreas. Little is known about the in vivo dynamic interactions between T cells and beta cells or the kinetic behavior of other immune cell subsets in the pancreatic islets. Utilizing multiphoton microscopy we have designed a technique that allows for the real-time visualization of diabetogenic T cells and dendritic cells in pancreatic islets in a live animal, including their interplay with beta cells and the vasculature. Using a custom designed stage, the pancreas was surgically exposed under live conditions so that imaging of islets under intact blood pressure and oxygen supply became possible. We demonstrate here that this approach allows for the tracking of diabetogenic leukocytes as well as vascularization phenotype of islets and accumulation of dendritic cells in islets during diabetes pathogenesis. This technique should be useful in mapping crucial kinetic events in T1D pathogenesis and in testing the impact of immune based interventions on T cell migration, extravasation and islet destruction. Public Library of Science 2010-12-23 /pmc/articles/PMC3009738/ /pubmed/21203413 http://dx.doi.org/10.1371/journal.pone.0015732 Text en Coppieters et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Coppieters, Ken
Martinic, Marianne M.
Kiosses, William B.
Amirian, Natalie
von Herrath, Matthias
A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title_full A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title_fullStr A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title_full_unstemmed A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title_short A Novel Technique for the In Vivo Imaging of Autoimmune Diabetes Development in the Pancreas by Two-Photon Microscopy
title_sort novel technique for the in vivo imaging of autoimmune diabetes development in the pancreas by two-photon microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3009738/
https://www.ncbi.nlm.nih.gov/pubmed/21203413
http://dx.doi.org/10.1371/journal.pone.0015732
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