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Quantitative analysis of the transcription control mechanism
Gene transcription requires a sequence of promoter state transitions, including chromatin remodeling, assembly of the transcription machinery, and clearance of the promoter by RNA polymerase. The rate-limiting steps in this sequence are regulated by transcriptional activators that bind at specific p...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
European Molecular Biology Organization
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010110/ https://www.ncbi.nlm.nih.gov/pubmed/21081924 http://dx.doi.org/10.1038/msb.2010.83 |
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author | Mao, Changhui Brown, Christopher R Falkovskaia, Elena Dong, Shawfeng Hrabeta-Robinson, Eva Wenger, Lauren Boeger, Hinrich |
author_facet | Mao, Changhui Brown, Christopher R Falkovskaia, Elena Dong, Shawfeng Hrabeta-Robinson, Eva Wenger, Lauren Boeger, Hinrich |
author_sort | Mao, Changhui |
collection | PubMed |
description | Gene transcription requires a sequence of promoter state transitions, including chromatin remodeling, assembly of the transcription machinery, and clearance of the promoter by RNA polymerase. The rate-limiting steps in this sequence are regulated by transcriptional activators that bind at specific promoter elements. As the transition kinetics of individual promoters cannot be observed, the identity of the activator-controlled steps has remained a matter of speculation. In this study, we investigated promoter chromatin structure, and the intrinsic noise of expression over a wide range of expression values for the PHO5 gene of yeast. Interpretation of our results with regard to a stochastic model of promoter chromatin remodeling and gene expression suggests that the regulatory architecture of the gene expression process is measurably reflected in its intrinsic noise profile. Our chromatin structure and noise analyses indicate that the activator of PHO5 transcription stimulates the rates of promoter nucleosome disassembly, and assembly of the transcription machinery after nucleosome removal, but no other rates of the expression process. |
format | Text |
id | pubmed-3010110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | European Molecular Biology Organization |
record_format | MEDLINE/PubMed |
spelling | pubmed-30101102010-12-27 Quantitative analysis of the transcription control mechanism Mao, Changhui Brown, Christopher R Falkovskaia, Elena Dong, Shawfeng Hrabeta-Robinson, Eva Wenger, Lauren Boeger, Hinrich Mol Syst Biol Article Gene transcription requires a sequence of promoter state transitions, including chromatin remodeling, assembly of the transcription machinery, and clearance of the promoter by RNA polymerase. The rate-limiting steps in this sequence are regulated by transcriptional activators that bind at specific promoter elements. As the transition kinetics of individual promoters cannot be observed, the identity of the activator-controlled steps has remained a matter of speculation. In this study, we investigated promoter chromatin structure, and the intrinsic noise of expression over a wide range of expression values for the PHO5 gene of yeast. Interpretation of our results with regard to a stochastic model of promoter chromatin remodeling and gene expression suggests that the regulatory architecture of the gene expression process is measurably reflected in its intrinsic noise profile. Our chromatin structure and noise analyses indicate that the activator of PHO5 transcription stimulates the rates of promoter nucleosome disassembly, and assembly of the transcription machinery after nucleosome removal, but no other rates of the expression process. European Molecular Biology Organization 2010-11-16 /pmc/articles/PMC3010110/ /pubmed/21081924 http://dx.doi.org/10.1038/msb.2010.83 Text en Copyright © 2010, EMBO and Macmillan Publishers Limited https://creativecommons.org/licenses/by-nc-sa/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Article Mao, Changhui Brown, Christopher R Falkovskaia, Elena Dong, Shawfeng Hrabeta-Robinson, Eva Wenger, Lauren Boeger, Hinrich Quantitative analysis of the transcription control mechanism |
title | Quantitative analysis of the transcription control mechanism |
title_full | Quantitative analysis of the transcription control mechanism |
title_fullStr | Quantitative analysis of the transcription control mechanism |
title_full_unstemmed | Quantitative analysis of the transcription control mechanism |
title_short | Quantitative analysis of the transcription control mechanism |
title_sort | quantitative analysis of the transcription control mechanism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010110/ https://www.ncbi.nlm.nih.gov/pubmed/21081924 http://dx.doi.org/10.1038/msb.2010.83 |
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