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Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene

The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, ho...

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Autores principales: Zong, Chenghang, So, Lok-hang, Sepúlveda, Leonardo A, Skinner, Samuel O, Golding, Ido
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010116/
https://www.ncbi.nlm.nih.gov/pubmed/21119634
http://dx.doi.org/10.1038/msb.2010.96
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author Zong, Chenghang
So, Lok-hang
Sepúlveda, Leonardo A
Skinner, Samuel O
Golding, Ido
author_facet Zong, Chenghang
So, Lok-hang
Sepúlveda, Leonardo A
Skinner, Samuel O
Golding, Ido
author_sort Zong, Chenghang
collection PubMed
description The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, how long will a cell stay in the lysogenic state before spontaneously switching away from that state? We show by direct measurement that lysogen stability exhibits a simple exponential dependence on the frequency of activity bursts from the fate-determining gene, cI. We quantify these gene-activity bursts using single-molecule-resolution mRNA measurements in individual cells, analyzed using a stochastic mathematical model of the gene-network kinetics. The quantitative relation between stability and gene activity is independent of the fine details of gene regulation, suggesting that a quantitative prediction of cell-state stability may also be possible in more complex systems.
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spelling pubmed-30101162010-12-27 Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene Zong, Chenghang So, Lok-hang Sepúlveda, Leonardo A Skinner, Samuel O Golding, Ido Mol Syst Biol Report The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, how long will a cell stay in the lysogenic state before spontaneously switching away from that state? We show by direct measurement that lysogen stability exhibits a simple exponential dependence on the frequency of activity bursts from the fate-determining gene, cI. We quantify these gene-activity bursts using single-molecule-resolution mRNA measurements in individual cells, analyzed using a stochastic mathematical model of the gene-network kinetics. The quantitative relation between stability and gene activity is independent of the fine details of gene regulation, suggesting that a quantitative prediction of cell-state stability may also be possible in more complex systems. Nature Publishing Group 2010-11-30 /pmc/articles/PMC3010116/ /pubmed/21119634 http://dx.doi.org/10.1038/msb.2010.96 Text en Copyright © 2010, EMBO and Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.
spellingShingle Report
Zong, Chenghang
So, Lok-hang
Sepúlveda, Leonardo A
Skinner, Samuel O
Golding, Ido
Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title_full Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title_fullStr Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title_full_unstemmed Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title_short Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
title_sort lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010116/
https://www.ncbi.nlm.nih.gov/pubmed/21119634
http://dx.doi.org/10.1038/msb.2010.96
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