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Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene
The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, ho...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010116/ https://www.ncbi.nlm.nih.gov/pubmed/21119634 http://dx.doi.org/10.1038/msb.2010.96 |
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author | Zong, Chenghang So, Lok-hang Sepúlveda, Leonardo A Skinner, Samuel O Golding, Ido |
author_facet | Zong, Chenghang So, Lok-hang Sepúlveda, Leonardo A Skinner, Samuel O Golding, Ido |
author_sort | Zong, Chenghang |
collection | PubMed |
description | The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, how long will a cell stay in the lysogenic state before spontaneously switching away from that state? We show by direct measurement that lysogen stability exhibits a simple exponential dependence on the frequency of activity bursts from the fate-determining gene, cI. We quantify these gene-activity bursts using single-molecule-resolution mRNA measurements in individual cells, analyzed using a stochastic mathematical model of the gene-network kinetics. The quantitative relation between stability and gene activity is independent of the fine details of gene regulation, suggesting that a quantitative prediction of cell-state stability may also be possible in more complex systems. |
format | Text |
id | pubmed-3010116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-30101162010-12-27 Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene Zong, Chenghang So, Lok-hang Sepúlveda, Leonardo A Skinner, Samuel O Golding, Ido Mol Syst Biol Report The ability of living cells to maintain an inheritable memory of their gene-expression state is key to cellular differentiation. Bacterial lysogeny serves as a simple paradigm for long-term cellular memory. In this study, we address the following question: in the absence of external perturbation, how long will a cell stay in the lysogenic state before spontaneously switching away from that state? We show by direct measurement that lysogen stability exhibits a simple exponential dependence on the frequency of activity bursts from the fate-determining gene, cI. We quantify these gene-activity bursts using single-molecule-resolution mRNA measurements in individual cells, analyzed using a stochastic mathematical model of the gene-network kinetics. The quantitative relation between stability and gene activity is independent of the fine details of gene regulation, suggesting that a quantitative prediction of cell-state stability may also be possible in more complex systems. Nature Publishing Group 2010-11-30 /pmc/articles/PMC3010116/ /pubmed/21119634 http://dx.doi.org/10.1038/msb.2010.96 Text en Copyright © 2010, EMBO and Macmillan Publishers Limited http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Report Zong, Chenghang So, Lok-hang Sepúlveda, Leonardo A Skinner, Samuel O Golding, Ido Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title | Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title_full | Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title_fullStr | Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title_full_unstemmed | Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title_short | Lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
title_sort | lysogen stability is determined by the frequency of activity bursts from the fate-determining gene |
topic | Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010116/ https://www.ncbi.nlm.nih.gov/pubmed/21119634 http://dx.doi.org/10.1038/msb.2010.96 |
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