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Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping

Electromechanical reshaping (EMR) has been recently described as an alternative method for reshaping facial cartilage without the need for incisions or sutures. This study focuses on determining the short- and long-term viability of chondrocytes following EMR in cartilage grafts maintained in tissue...

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Detalles Bibliográficos
Autores principales: Protsenko, Dmitry E., Ho, Kevin, Wong, Brian J. F.
Formato: Texto
Lenguaje:English
Publicado: Springer US 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010201/
https://www.ncbi.nlm.nih.gov/pubmed/20842431
http://dx.doi.org/10.1007/s10439-010-0139-7
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author Protsenko, Dmitry E.
Ho, Kevin
Wong, Brian J. F.
author_facet Protsenko, Dmitry E.
Ho, Kevin
Wong, Brian J. F.
author_sort Protsenko, Dmitry E.
collection PubMed
description Electromechanical reshaping (EMR) has been recently described as an alternative method for reshaping facial cartilage without the need for incisions or sutures. This study focuses on determining the short- and long-term viability of chondrocytes following EMR in cartilage grafts maintained in tissue culture. Flat rabbit nasal septal cartilage specimens were bent into semi-cylindrical shapes by an aluminum jig while a constant electric voltage was applied across the concave and convex surfaces. After EMR, specimens were maintained in culture media for 64 days. Over this time period, specimens were serially biopsied and then stained with a fluorescent live–dead assay system and imaged using laser scanning confocal microscopy. In addition, the fraction of viable chondrocytes was measured, correlated with voltage, voltage application time, electric field configuration, and examined serially. The fraction of viable chondrocytes decreased with voltage and application time. High local electric field intensity and proximity to the positive electrode also focally reduced chondrocyte viability. The density of viable chondrocytes decreased over time and reached a steady state after 2–4 weeks. Viable cells were concentrated within the central region of the specimen. Approximately 20% of original chondrocytes remained viable after reshaping with optimal voltage and application time parameters and compared favorably with conventional surgical shape change techniques such as morselization.
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spelling pubmed-30102012011-01-19 Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping Protsenko, Dmitry E. Ho, Kevin Wong, Brian J. F. Ann Biomed Eng Article Electromechanical reshaping (EMR) has been recently described as an alternative method for reshaping facial cartilage without the need for incisions or sutures. This study focuses on determining the short- and long-term viability of chondrocytes following EMR in cartilage grafts maintained in tissue culture. Flat rabbit nasal septal cartilage specimens were bent into semi-cylindrical shapes by an aluminum jig while a constant electric voltage was applied across the concave and convex surfaces. After EMR, specimens were maintained in culture media for 64 days. Over this time period, specimens were serially biopsied and then stained with a fluorescent live–dead assay system and imaged using laser scanning confocal microscopy. In addition, the fraction of viable chondrocytes was measured, correlated with voltage, voltage application time, electric field configuration, and examined serially. The fraction of viable chondrocytes decreased with voltage and application time. High local electric field intensity and proximity to the positive electrode also focally reduced chondrocyte viability. The density of viable chondrocytes decreased over time and reached a steady state after 2–4 weeks. Viable cells were concentrated within the central region of the specimen. Approximately 20% of original chondrocytes remained viable after reshaping with optimal voltage and application time parameters and compared favorably with conventional surgical shape change techniques such as morselization. Springer US 2010-09-15 2011 /pmc/articles/PMC3010201/ /pubmed/20842431 http://dx.doi.org/10.1007/s10439-010-0139-7 Text en © The Author(s) 2010 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Protsenko, Dmitry E.
Ho, Kevin
Wong, Brian J. F.
Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title_full Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title_fullStr Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title_full_unstemmed Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title_short Survival of Chondrocytes in Rabbit Septal Cartilage After Electromechanical Reshaping
title_sort survival of chondrocytes in rabbit septal cartilage after electromechanical reshaping
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010201/
https://www.ncbi.nlm.nih.gov/pubmed/20842431
http://dx.doi.org/10.1007/s10439-010-0139-7
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