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Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells
OBJECTIVES: To determine whether a novel marine micro-organism with anticancer properties, H31, the metabolic product of Bacillus SW31, has anti-tumor effects on head and neck cancer, and potential for apoptotic-enhancing anti-cancer treatment of affected patients. METHODS: The cell viability and ap...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Korean Society of Otorhinolaryngology-Head and Neck Surgery
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010542/ https://www.ncbi.nlm.nih.gov/pubmed/21217964 http://dx.doi.org/10.3342/ceo.2010.3.4.217 |
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author | Lim, Young Chang Cho, Ki Woong Kwon, Hak Cheol Kang, Sung Un Pyun, Jung Hee Lee, Mi Hye Hwang, Hye Sook Kim, Jang Hee Lee, Ha Neul Choi, Eun Chang Kim, Chul-Ho |
author_facet | Lim, Young Chang Cho, Ki Woong Kwon, Hak Cheol Kang, Sung Un Pyun, Jung Hee Lee, Mi Hye Hwang, Hye Sook Kim, Jang Hee Lee, Ha Neul Choi, Eun Chang Kim, Chul-Ho |
author_sort | Lim, Young Chang |
collection | PubMed |
description | OBJECTIVES: To determine whether a novel marine micro-organism with anticancer properties, H31, the metabolic product of Bacillus SW31, has anti-tumor effects on head and neck cancer, and potential for apoptotic-enhancing anti-cancer treatment of affected patients. METHODS: The cell viability and apoptosis assays were performed. Changes in the signal pathway related to apoptosis were investigated. Then, the therapeutic effects of H31 were explored in mouse xenograft model and drug toxicity of H31 was examined in zebrafish model. RESULTS: We identified the anticancer activity of H31, a novel metabolic product of Bacillus SW31. Bacillus SW31, a new marine micro-organism, has 70% homology with Bacillus firmus and contains potent cytotoxic bioactivity in head and neck cancer cells using MTT assay. Combined with c-JUN, p53, cytochrome C, and caspase-3, H31 induced apoptosis of KB cells, a head and neck cancer cell line. In a separate in vivo model, tumor growth in C3H/HeJ syngeneic mice was suppressed by H31. In addition, in a zebrafish model used for toxicity testing, a considerable dose of H31 did not result in embryo or neurotoxicity. CONCLUSION: Growth inhibition and apoptosis were achieved both in vitro and in vivo in head and neck cancer cells after exposure to H31, a metabolite from the marine Bacillus species, without any significant toxicity effects even at considerable H31 dose concentrations. |
format | Text |
id | pubmed-3010542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Korean Society of Otorhinolaryngology-Head and Neck Surgery |
record_format | MEDLINE/PubMed |
spelling | pubmed-30105422011-01-07 Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells Lim, Young Chang Cho, Ki Woong Kwon, Hak Cheol Kang, Sung Un Pyun, Jung Hee Lee, Mi Hye Hwang, Hye Sook Kim, Jang Hee Lee, Ha Neul Choi, Eun Chang Kim, Chul-Ho Clin Exp Otorhinolaryngol Original Article OBJECTIVES: To determine whether a novel marine micro-organism with anticancer properties, H31, the metabolic product of Bacillus SW31, has anti-tumor effects on head and neck cancer, and potential for apoptotic-enhancing anti-cancer treatment of affected patients. METHODS: The cell viability and apoptosis assays were performed. Changes in the signal pathway related to apoptosis were investigated. Then, the therapeutic effects of H31 were explored in mouse xenograft model and drug toxicity of H31 was examined in zebrafish model. RESULTS: We identified the anticancer activity of H31, a novel metabolic product of Bacillus SW31. Bacillus SW31, a new marine micro-organism, has 70% homology with Bacillus firmus and contains potent cytotoxic bioactivity in head and neck cancer cells using MTT assay. Combined with c-JUN, p53, cytochrome C, and caspase-3, H31 induced apoptosis of KB cells, a head and neck cancer cell line. In a separate in vivo model, tumor growth in C3H/HeJ syngeneic mice was suppressed by H31. In addition, in a zebrafish model used for toxicity testing, a considerable dose of H31 did not result in embryo or neurotoxicity. CONCLUSION: Growth inhibition and apoptosis were achieved both in vitro and in vivo in head and neck cancer cells after exposure to H31, a metabolite from the marine Bacillus species, without any significant toxicity effects even at considerable H31 dose concentrations. Korean Society of Otorhinolaryngology-Head and Neck Surgery 2010-12 2010-12-22 /pmc/articles/PMC3010542/ /pubmed/21217964 http://dx.doi.org/10.3342/ceo.2010.3.4.217 Text en Copyright © 2010 by Korean Society of Otorhinolaryngology-Head and Neck Surgery. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Lim, Young Chang Cho, Ki Woong Kwon, Hak Cheol Kang, Sung Un Pyun, Jung Hee Lee, Mi Hye Hwang, Hye Sook Kim, Jang Hee Lee, Ha Neul Choi, Eun Chang Kim, Chul-Ho Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title | Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title_full | Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title_fullStr | Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title_full_unstemmed | Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title_short | Growth Inhibition and Apoptosis with H31 Metabolites from Marine Bacillus SW31 in Head and Neck Cancer Cells |
title_sort | growth inhibition and apoptosis with h31 metabolites from marine bacillus sw31 in head and neck cancer cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010542/ https://www.ncbi.nlm.nih.gov/pubmed/21217964 http://dx.doi.org/10.3342/ceo.2010.3.4.217 |
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