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Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy

Dendritic cells (DCs) are the most potent antigen-presenting cells. They play a vital role in the initiation of immune response by presenting antigens to T cells and followed by induction of T-cell response. Reported research in animal studies indicated that vaccine immunity could be a promising alt...

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Autores principales: Chen, Yu-Zhe, Yao, Xing-Lei, Tabata, Yasuhiko, Nakagawa, Shinsaku, Gao, Jian-Qing
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010860/
https://www.ncbi.nlm.nih.gov/pubmed/21197274
http://dx.doi.org/10.1155/2010/565643
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author Chen, Yu-Zhe
Yao, Xing-Lei
Tabata, Yasuhiko
Nakagawa, Shinsaku
Gao, Jian-Qing
author_facet Chen, Yu-Zhe
Yao, Xing-Lei
Tabata, Yasuhiko
Nakagawa, Shinsaku
Gao, Jian-Qing
author_sort Chen, Yu-Zhe
collection PubMed
description Dendritic cells (DCs) are the most potent antigen-presenting cells. They play a vital role in the initiation of immune response by presenting antigens to T cells and followed by induction of T-cell response. Reported research in animal studies indicated that vaccine immunity could be a promising alternative therapy for cancer patients. However, broad clinical utility has not been achieved yet, owing to the low transfection efficiency of DCs. Therefore, it is essential to improve the transfection efficiency of DC-based vaccination in immunotherapy. In several studies, DCs were genetically engineered by tumor-associated antigens or by immune molecules such as costimulatory molecules, cytokines, and chemokines. Encouraging results have been achieved in cancer treatment using various animal models. This paper describes the recent progress in gene delivery systems including viral vectors and nonviral carriers for DC-based genetically engineered vaccines. The reverse and three-dimensional transfection systems developed in DCs are also discussed.
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spelling pubmed-30108602010-12-30 Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy Chen, Yu-Zhe Yao, Xing-Lei Tabata, Yasuhiko Nakagawa, Shinsaku Gao, Jian-Qing Clin Dev Immunol Review Article Dendritic cells (DCs) are the most potent antigen-presenting cells. They play a vital role in the initiation of immune response by presenting antigens to T cells and followed by induction of T-cell response. Reported research in animal studies indicated that vaccine immunity could be a promising alternative therapy for cancer patients. However, broad clinical utility has not been achieved yet, owing to the low transfection efficiency of DCs. Therefore, it is essential to improve the transfection efficiency of DC-based vaccination in immunotherapy. In several studies, DCs were genetically engineered by tumor-associated antigens or by immune molecules such as costimulatory molecules, cytokines, and chemokines. Encouraging results have been achieved in cancer treatment using various animal models. This paper describes the recent progress in gene delivery systems including viral vectors and nonviral carriers for DC-based genetically engineered vaccines. The reverse and three-dimensional transfection systems developed in DCs are also discussed. Hindawi Publishing Corporation 2010 2010-12-20 /pmc/articles/PMC3010860/ /pubmed/21197274 http://dx.doi.org/10.1155/2010/565643 Text en Copyright © 2010 Yu-Zhe Chen et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Chen, Yu-Zhe
Yao, Xing-Lei
Tabata, Yasuhiko
Nakagawa, Shinsaku
Gao, Jian-Qing
Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title_full Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title_fullStr Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title_full_unstemmed Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title_short Gene Carriers and Transfection Systems Used in the Recombination of Dendritic Cells for Effective Cancer Immunotherapy
title_sort gene carriers and transfection systems used in the recombination of dendritic cells for effective cancer immunotherapy
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010860/
https://www.ncbi.nlm.nih.gov/pubmed/21197274
http://dx.doi.org/10.1155/2010/565643
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