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Translational Up-Regulation and High-Level Protein Expression from Plasmid Vectors by mTOR Activation via Different Pathways in PC3 and 293T Cells

BACKGROUND: Though 293T cells are widely used for expression of proteins from transfected plasmid vectors, the molecular basis for the high-level expression is yet to be understood. We recently identified the prostate carcinoma cell line PC3 to be as efficient as 293T in protein expression. This stu...

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Detalles Bibliográficos
Autores principales: Karyala, Prashanthi, Namsa, Nima D., Chilakalapudi, Durga Rao
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010991/
https://www.ncbi.nlm.nih.gov/pubmed/21203441
http://dx.doi.org/10.1371/journal.pone.0014408