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Translational Up-Regulation and High-Level Protein Expression from Plasmid Vectors by mTOR Activation via Different Pathways in PC3 and 293T Cells
BACKGROUND: Though 293T cells are widely used for expression of proteins from transfected plasmid vectors, the molecular basis for the high-level expression is yet to be understood. We recently identified the prostate carcinoma cell line PC3 to be as efficient as 293T in protein expression. This stu...
Autores principales: | Karyala, Prashanthi, Namsa, Nima D., Chilakalapudi, Durga Rao |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3010991/ https://www.ncbi.nlm.nih.gov/pubmed/21203441 http://dx.doi.org/10.1371/journal.pone.0014408 |
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