Cargando…

TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans

C. elegans first stage (L1) larvae hatched in the absence of food, arrest development and enter an L1 diapause, whereby they can survive starvation for several weeks. The physiological and metabolic requirements for survival during L1 diapause are poorly understood. However, yolk, a cholesterol bind...

Descripción completa

Detalles Bibliográficos
Autores principales: Chotard, Laëtitia, Skorobogata, Olga, Sylvain, Marc-André, Shrivastava, Sanhita, Rocheleau, Christian E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011002/
https://www.ncbi.nlm.nih.gov/pubmed/21203392
http://dx.doi.org/10.1371/journal.pone.0015662
_version_ 1782194866910920704
author Chotard, Laëtitia
Skorobogata, Olga
Sylvain, Marc-André
Shrivastava, Sanhita
Rocheleau, Christian E.
author_facet Chotard, Laëtitia
Skorobogata, Olga
Sylvain, Marc-André
Shrivastava, Sanhita
Rocheleau, Christian E.
author_sort Chotard, Laëtitia
collection PubMed
description C. elegans first stage (L1) larvae hatched in the absence of food, arrest development and enter an L1 diapause, whereby they can survive starvation for several weeks. The physiological and metabolic requirements for survival during L1 diapause are poorly understood. However, yolk, a cholesterol binding/transport protein, has been suggested to serve as an energy source. Here, we demonstrate that C. elegans TBC-2, a RAB-5 GTPase Activating Protein (GAP) involved in early-to-late endosome transition, is important for yolk protein storage during embryogenesis and for L1 survival during starvation. We found during embryogenesis, that a yolk::green fluorescent protein fusion (YP170::GFP), disappeared much more quickly in tbc-2 mutant embryos as compared with wild-type control embryos. The premature disappearance of YP170::GFP in tbc-2 mutants is likely due to premature degradation in the lysosomes as we found that YP170::GFP showed increased colocalization with Lysotracker Red, a marker for acidic compartments. Furthermore, YP170::GFP disappearance in tbc-2 mutants required RAB-7, a regulator of endosome to lysosome trafficking. Although tbc-2 is not essential in fed animals, we discovered that tbc-2 mutant L1 larvae have strongly reduced survival when hatched in the absence of food. We show that tbc-2 mutant larvae are not defective in maintaining L1 diapause and that mutants defective in yolk uptake, rme-1 and rme-6, also had strongly reduced L1 survival when hatched in the absence of food. Our findings demonstrate that TBC-2 is required for yolk protein storage during embryonic development and provide strong correlative data indicating that yolk constitutes an important energy source for larval survival during L1 diapause.
format Text
id pubmed-3011002
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30110022011-01-03 TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans Chotard, Laëtitia Skorobogata, Olga Sylvain, Marc-André Shrivastava, Sanhita Rocheleau, Christian E. PLoS One Research Article C. elegans first stage (L1) larvae hatched in the absence of food, arrest development and enter an L1 diapause, whereby they can survive starvation for several weeks. The physiological and metabolic requirements for survival during L1 diapause are poorly understood. However, yolk, a cholesterol binding/transport protein, has been suggested to serve as an energy source. Here, we demonstrate that C. elegans TBC-2, a RAB-5 GTPase Activating Protein (GAP) involved in early-to-late endosome transition, is important for yolk protein storage during embryogenesis and for L1 survival during starvation. We found during embryogenesis, that a yolk::green fluorescent protein fusion (YP170::GFP), disappeared much more quickly in tbc-2 mutant embryos as compared with wild-type control embryos. The premature disappearance of YP170::GFP in tbc-2 mutants is likely due to premature degradation in the lysosomes as we found that YP170::GFP showed increased colocalization with Lysotracker Red, a marker for acidic compartments. Furthermore, YP170::GFP disappearance in tbc-2 mutants required RAB-7, a regulator of endosome to lysosome trafficking. Although tbc-2 is not essential in fed animals, we discovered that tbc-2 mutant L1 larvae have strongly reduced survival when hatched in the absence of food. We show that tbc-2 mutant larvae are not defective in maintaining L1 diapause and that mutants defective in yolk uptake, rme-1 and rme-6, also had strongly reduced L1 survival when hatched in the absence of food. Our findings demonstrate that TBC-2 is required for yolk protein storage during embryonic development and provide strong correlative data indicating that yolk constitutes an important energy source for larval survival during L1 diapause. Public Library of Science 2010-12-28 /pmc/articles/PMC3011002/ /pubmed/21203392 http://dx.doi.org/10.1371/journal.pone.0015662 Text en Chotard et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Chotard, Laëtitia
Skorobogata, Olga
Sylvain, Marc-André
Shrivastava, Sanhita
Rocheleau, Christian E.
TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title_full TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title_fullStr TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title_full_unstemmed TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title_short TBC-2 Is Required for Embryonic Yolk Protein Storage and Larval Survival during L1 Diapause in Caenorhabditis elegans
title_sort tbc-2 is required for embryonic yolk protein storage and larval survival during l1 diapause in caenorhabditis elegans
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011002/
https://www.ncbi.nlm.nih.gov/pubmed/21203392
http://dx.doi.org/10.1371/journal.pone.0015662
work_keys_str_mv AT chotardlaetitia tbc2isrequiredforembryonicyolkproteinstorageandlarvalsurvivalduringl1diapauseincaenorhabditiselegans
AT skorobogataolga tbc2isrequiredforembryonicyolkproteinstorageandlarvalsurvivalduringl1diapauseincaenorhabditiselegans
AT sylvainmarcandre tbc2isrequiredforembryonicyolkproteinstorageandlarvalsurvivalduringl1diapauseincaenorhabditiselegans
AT shrivastavasanhita tbc2isrequiredforembryonicyolkproteinstorageandlarvalsurvivalduringl1diapauseincaenorhabditiselegans
AT rocheleauchristiane tbc2isrequiredforembryonicyolkproteinstorageandlarvalsurvivalduringl1diapauseincaenorhabditiselegans