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Growth and Differentiation of the Larval Mosquito Midgut
Factors affecting larval growth and nutrition have consequences on adult fecundity. Since the mosquito larval midgut is the primary organ of digestion and nutrient absorption, factors that affect the growth and development of the midgut may have potential consequences on the reproductive potential o...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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University of Wisconsin Library
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011905/ https://www.ncbi.nlm.nih.gov/pubmed/20053117 http://dx.doi.org/10.1673/031.009.5501 |
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author | Ray, Kathryn Mercedes, Maria Chan, Doris Choi, Chi Yan Nishiura, James T. |
author_facet | Ray, Kathryn Mercedes, Maria Chan, Doris Choi, Chi Yan Nishiura, James T. |
author_sort | Ray, Kathryn |
collection | PubMed |
description | Factors affecting larval growth and nutrition have consequences on adult fecundity. Since the mosquito larval midgut is the primary organ of digestion and nutrient absorption, factors that affect the growth and development of the midgut may have potential consequences on the reproductive potential of the adult. To gain a better understanding of mosquito midgut development the growth and metamorphic remodeling of the Aedes aegypti L. and Culex pipiens L. (Diptera: Culicidae) midguts were investigated. Cytological evidence was obtained suggesting that, in both the anterior and posterior Ae. aegypti larval midgut, diploid regenerative cells give rise to new endoreplicating cells that significantly contribute to the growth and metabolism of the midgut. This hypothesis was supported by BrdU incorporation studies showing that diploid cells, as well as large and small endoreplicating cells, synthesize DNA during the 2(nd), 3(rd) and 4(th) instars. Cytological studies of the Cx. pipiens larval midgut suggest that anterior midgut growth in this species is primarily by cell enlargement. To study metamorphic remodeling of the midgut, DNA synthesis in Ae. aegypti 4(th) instar midguts was followed by using 5-bromo-2-deoxyuridine (BrdU) incorporation. During the 24 hr period after the last larval-larval molt both endoreplicating and diploid cells incorporate BrdU. After the critical weight is achieved, endoreplicating cell BrdU incorporation gradually ceases while diploid cells continue to replicate. The period of maximum diploid cell incorporation correlated with the period of maximum ecdysone titer. |
format | Text |
id | pubmed-3011905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | University of Wisconsin Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-30119052011-09-01 Growth and Differentiation of the Larval Mosquito Midgut Ray, Kathryn Mercedes, Maria Chan, Doris Choi, Chi Yan Nishiura, James T. J Insect Sci Article Factors affecting larval growth and nutrition have consequences on adult fecundity. Since the mosquito larval midgut is the primary organ of digestion and nutrient absorption, factors that affect the growth and development of the midgut may have potential consequences on the reproductive potential of the adult. To gain a better understanding of mosquito midgut development the growth and metamorphic remodeling of the Aedes aegypti L. and Culex pipiens L. (Diptera: Culicidae) midguts were investigated. Cytological evidence was obtained suggesting that, in both the anterior and posterior Ae. aegypti larval midgut, diploid regenerative cells give rise to new endoreplicating cells that significantly contribute to the growth and metabolism of the midgut. This hypothesis was supported by BrdU incorporation studies showing that diploid cells, as well as large and small endoreplicating cells, synthesize DNA during the 2(nd), 3(rd) and 4(th) instars. Cytological studies of the Cx. pipiens larval midgut suggest that anterior midgut growth in this species is primarily by cell enlargement. To study metamorphic remodeling of the midgut, DNA synthesis in Ae. aegypti 4(th) instar midguts was followed by using 5-bromo-2-deoxyuridine (BrdU) incorporation. During the 24 hr period after the last larval-larval molt both endoreplicating and diploid cells incorporate BrdU. After the critical weight is achieved, endoreplicating cell BrdU incorporation gradually ceases while diploid cells continue to replicate. The period of maximum diploid cell incorporation correlated with the period of maximum ecdysone titer. University of Wisconsin Library 2009-07-15 /pmc/articles/PMC3011905/ /pubmed/20053117 http://dx.doi.org/10.1673/031.009.5501 Text en © 2009 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Ray, Kathryn Mercedes, Maria Chan, Doris Choi, Chi Yan Nishiura, James T. Growth and Differentiation of the Larval Mosquito Midgut |
title | Growth and Differentiation of the Larval Mosquito Midgut |
title_full | Growth and Differentiation of the Larval Mosquito Midgut |
title_fullStr | Growth and Differentiation of the Larval Mosquito Midgut |
title_full_unstemmed | Growth and Differentiation of the Larval Mosquito Midgut |
title_short | Growth and Differentiation of the Larval Mosquito Midgut |
title_sort | growth and differentiation of the larval mosquito midgut |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3011905/ https://www.ncbi.nlm.nih.gov/pubmed/20053117 http://dx.doi.org/10.1673/031.009.5501 |
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