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Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation
BACKGROUND: Anchorage independent growth is an important hallmark of oncogenic transformation. Previous studies have shown that when adhesion dependent fibroblasts were prevented from adhering to a substrate they underwent anoikis. In the present study we have demonstrated how anoikis resistant cell...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012028/ https://www.ncbi.nlm.nih.gov/pubmed/21122158 http://dx.doi.org/10.1186/1471-2121-11-93 |
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author | Jinka, Rajeswari Kapoor, Renu Pavuluri, Sivapriya Raj, Avinash T Kumar, Mahesh J Rao, Lakshmi Pande, Gopal |
author_facet | Jinka, Rajeswari Kapoor, Renu Pavuluri, Sivapriya Raj, Avinash T Kumar, Mahesh J Rao, Lakshmi Pande, Gopal |
author_sort | Jinka, Rajeswari |
collection | PubMed |
description | BACKGROUND: Anchorage independent growth is an important hallmark of oncogenic transformation. Previous studies have shown that when adhesion dependent fibroblasts were prevented from adhering to a substrate they underwent anoikis. In the present study we have demonstrated how anoikis resistant cells gain the transformation related properties with sequential selection of genes. We have proposed this process as a model system for selection of transformed cells from normal cells. RESULTS: This report demonstrates that some fibroblasts can survive during late stages of anoikis, at which time they exhibit transformation-associated properties such as in vitro colony formation in soft agar and in vivo subcutaneous tumour formation in nude mice. Cytogenetic characterisation of these cells revealed that they contained a t (2; 2) derivative chromosome and they have a selective survival advantage in non adherent conditions. Gene expression profile indicated that these cells over expressed genes related to hypoxia, glycolysis and tumor suppression/metastasis which could be helpful in their retaining a transformed phenotype. CONCLUSION: Our results reveal some new links between anoikis and cell transformation and they provide a reproducible model system which can potentially be useful to study multistage cancer and to identify new targets for drug development. |
format | Text |
id | pubmed-3012028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30120282010-12-30 Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation Jinka, Rajeswari Kapoor, Renu Pavuluri, Sivapriya Raj, Avinash T Kumar, Mahesh J Rao, Lakshmi Pande, Gopal BMC Cell Biol Research Article BACKGROUND: Anchorage independent growth is an important hallmark of oncogenic transformation. Previous studies have shown that when adhesion dependent fibroblasts were prevented from adhering to a substrate they underwent anoikis. In the present study we have demonstrated how anoikis resistant cells gain the transformation related properties with sequential selection of genes. We have proposed this process as a model system for selection of transformed cells from normal cells. RESULTS: This report demonstrates that some fibroblasts can survive during late stages of anoikis, at which time they exhibit transformation-associated properties such as in vitro colony formation in soft agar and in vivo subcutaneous tumour formation in nude mice. Cytogenetic characterisation of these cells revealed that they contained a t (2; 2) derivative chromosome and they have a selective survival advantage in non adherent conditions. Gene expression profile indicated that these cells over expressed genes related to hypoxia, glycolysis and tumor suppression/metastasis which could be helpful in their retaining a transformed phenotype. CONCLUSION: Our results reveal some new links between anoikis and cell transformation and they provide a reproducible model system which can potentially be useful to study multistage cancer and to identify new targets for drug development. BioMed Central 2010-12-02 /pmc/articles/PMC3012028/ /pubmed/21122158 http://dx.doi.org/10.1186/1471-2121-11-93 Text en Copyright ©2010 Jinka et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Jinka, Rajeswari Kapoor, Renu Pavuluri, Sivapriya Raj, Avinash T Kumar, Mahesh J Rao, Lakshmi Pande, Gopal Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title | Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title_full | Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title_fullStr | Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title_full_unstemmed | Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title_short | Differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
title_sort | differential gene expression and clonal selection during cellular transformation induced by adhesion deprivation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012028/ https://www.ncbi.nlm.nih.gov/pubmed/21122158 http://dx.doi.org/10.1186/1471-2121-11-93 |
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