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Overexpression of Pim-1 in bladder cancer
BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly underst...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012037/ https://www.ncbi.nlm.nih.gov/pubmed/21143989 http://dx.doi.org/10.1186/1756-9966-29-161 |
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author | Guo, Shengjie Mao, Xiaopeng Chen, Junxing Huang, Bin Jin, Chu Xu, Zhenbo Qiu, Shaopeng |
author_facet | Guo, Shengjie Mao, Xiaopeng Chen, Junxing Huang, Bin Jin, Chu Xu, Zhenbo Qiu, Shaopeng |
author_sort | Guo, Shengjie |
collection | PubMed |
description | BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly understood. METHODS: Expression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer, its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA, and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays. RESULTS: When compared with normal epithelium, Pim-1 was overexpressed in bladder cancer epithelium, and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover, the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugs in vitro. CONCLUSIONS: Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer. |
format | Text |
id | pubmed-3012037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30120372010-12-30 Overexpression of Pim-1 in bladder cancer Guo, Shengjie Mao, Xiaopeng Chen, Junxing Huang, Bin Jin, Chu Xu, Zhenbo Qiu, Shaopeng J Exp Clin Cancer Res Research BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly understood. METHODS: Expression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer, its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA, and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays. RESULTS: When compared with normal epithelium, Pim-1 was overexpressed in bladder cancer epithelium, and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover, the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugs in vitro. CONCLUSIONS: Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer. BioMed Central 2010-12-11 /pmc/articles/PMC3012037/ /pubmed/21143989 http://dx.doi.org/10.1186/1756-9966-29-161 Text en Copyright ©2010 Guo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Guo, Shengjie Mao, Xiaopeng Chen, Junxing Huang, Bin Jin, Chu Xu, Zhenbo Qiu, Shaopeng Overexpression of Pim-1 in bladder cancer |
title | Overexpression of Pim-1 in bladder cancer |
title_full | Overexpression of Pim-1 in bladder cancer |
title_fullStr | Overexpression of Pim-1 in bladder cancer |
title_full_unstemmed | Overexpression of Pim-1 in bladder cancer |
title_short | Overexpression of Pim-1 in bladder cancer |
title_sort | overexpression of pim-1 in bladder cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012037/ https://www.ncbi.nlm.nih.gov/pubmed/21143989 http://dx.doi.org/10.1186/1756-9966-29-161 |
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