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Overexpression of Pim-1 in bladder cancer

BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly underst...

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Autores principales: Guo, Shengjie, Mao, Xiaopeng, Chen, Junxing, Huang, Bin, Jin, Chu, Xu, Zhenbo, Qiu, Shaopeng
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012037/
https://www.ncbi.nlm.nih.gov/pubmed/21143989
http://dx.doi.org/10.1186/1756-9966-29-161
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author Guo, Shengjie
Mao, Xiaopeng
Chen, Junxing
Huang, Bin
Jin, Chu
Xu, Zhenbo
Qiu, Shaopeng
author_facet Guo, Shengjie
Mao, Xiaopeng
Chen, Junxing
Huang, Bin
Jin, Chu
Xu, Zhenbo
Qiu, Shaopeng
author_sort Guo, Shengjie
collection PubMed
description BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly understood. METHODS: Expression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer, its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA, and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays. RESULTS: When compared with normal epithelium, Pim-1 was overexpressed in bladder cancer epithelium, and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover, the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugs in vitro. CONCLUSIONS: Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer.
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spelling pubmed-30120372010-12-30 Overexpression of Pim-1 in bladder cancer Guo, Shengjie Mao, Xiaopeng Chen, Junxing Huang, Bin Jin, Chu Xu, Zhenbo Qiu, Shaopeng J Exp Clin Cancer Res Research BACKGROUND: Pim-1 is a serine-threonine kinase which promotes early transformation, cell proliferation and cell survival during tumorigenesis. Several studies have demonstrated that Pim-1 kinase play a role in different cancer types, however, the function of Pim-1 in bladder cancer is poorly understood. METHODS: Expression and localization of Pim-1 in human normal and malignant bladder specimens were examined by Immunohistochemistry and Pim-1 staining score was compared with several clinicopathologic parameters. To further demonstrate the biological function of Pim-1 in bladder cancer, its expression was validated in five bladder cancer cell lines by western blot and immunohistochemistry analyses. Subsequent knockdown of Pim-1 was achieved by lentivirus encoding small interfering RNA, and the effect of Pim-1 on bladder cell survival and drug sensitivity were further assessed by colony formation and cell proliferation assays. RESULTS: When compared with normal epithelium, Pim-1 was overexpressed in bladder cancer epithelium, and the expression level was higher in invasive bladder cancer than Non-invasive bladder cancer specimens. Pim-1 was also detected in all the bladder cancer cell lines examined in our study. Moreover, the knockdown of Pim-1 significantly inhibited bladder cancer cell growth and also sensitized cells to chemotherapeutic drugs in vitro. CONCLUSIONS: Our results in this study suggest that Pim-1 may play a role in bladder cancer initiation and progression. Since Pim-1 is also involved in bladder cancer cell survival and drug resistance, Pim-1 is a potential candidate for targeted therapy in bladder cancer. BioMed Central 2010-12-11 /pmc/articles/PMC3012037/ /pubmed/21143989 http://dx.doi.org/10.1186/1756-9966-29-161 Text en Copyright ©2010 Guo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Guo, Shengjie
Mao, Xiaopeng
Chen, Junxing
Huang, Bin
Jin, Chu
Xu, Zhenbo
Qiu, Shaopeng
Overexpression of Pim-1 in bladder cancer
title Overexpression of Pim-1 in bladder cancer
title_full Overexpression of Pim-1 in bladder cancer
title_fullStr Overexpression of Pim-1 in bladder cancer
title_full_unstemmed Overexpression of Pim-1 in bladder cancer
title_short Overexpression of Pim-1 in bladder cancer
title_sort overexpression of pim-1 in bladder cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012037/
https://www.ncbi.nlm.nih.gov/pubmed/21143989
http://dx.doi.org/10.1186/1756-9966-29-161
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