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Analysis of MicroRNA Expression in the Prepubertal Testis
Only thirteen microRNAs are conserved between D. melanogaster and the mouse; however, conditional loss of miRNA function through mutation of Dicer causes defects in proliferation of premeiotic germ cells in both species. This highlights the potentially important, but uncharacterized, role of miRNAs...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012074/ https://www.ncbi.nlm.nih.gov/pubmed/21206922 http://dx.doi.org/10.1371/journal.pone.0015317 |
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author | Buchold, Gregory M. Coarfa, Cristian Kim, Jong Milosavljevic, Aleksandar Gunaratne, Preethi H. Matzuk, Martin M. |
author_facet | Buchold, Gregory M. Coarfa, Cristian Kim, Jong Milosavljevic, Aleksandar Gunaratne, Preethi H. Matzuk, Martin M. |
author_sort | Buchold, Gregory M. |
collection | PubMed |
description | Only thirteen microRNAs are conserved between D. melanogaster and the mouse; however, conditional loss of miRNA function through mutation of Dicer causes defects in proliferation of premeiotic germ cells in both species. This highlights the potentially important, but uncharacterized, role of miRNAs during early spermatogenesis. The goal of this study was to characterize on postnatal day 7, 10, and 14 the content and editing of murine testicular miRNAs, which predominantly arise from spermatogonia and spermatocytes, in contrast to prior descriptions of miRNAs in the adult mouse testis which largely reflects the content of spermatids. Previous studies have shown miRNAs to be abundant in the mouse testis by postnatal day 14; however, through Next Generation Sequencing of testes from a B6;129 background we found abundant earlier expression of miRNAs and describe shifts in the miRNA signature during this period. We detected robust expression of miRNAs encoded on the X chromosome in postnatal day 14 testes, consistent with prior studies showing their resistance to meiotic sex chromosome inactivation. Unexpectedly, we also found a similar positional enrichment for most miRNAs on chromosome 2 at postnatal day 14 and for those on chromosome 12 at postnatal day 7. We quantified in vivo developmental changes in three types of miRNA variation including 5′ heterogeneity, editing, and 3′ nucleotide addition. We identified eleven putative novel pubertal testis miRNAs whose developmental expression suggests a possible role in early male germ cell development. These studies provide a foundation for interpretation of miRNA changes associated with testicular pathology and identification of novel components of the miRNA editing machinery in the testis. |
format | Text |
id | pubmed-3012074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30120742011-01-04 Analysis of MicroRNA Expression in the Prepubertal Testis Buchold, Gregory M. Coarfa, Cristian Kim, Jong Milosavljevic, Aleksandar Gunaratne, Preethi H. Matzuk, Martin M. PLoS One Research Article Only thirteen microRNAs are conserved between D. melanogaster and the mouse; however, conditional loss of miRNA function through mutation of Dicer causes defects in proliferation of premeiotic germ cells in both species. This highlights the potentially important, but uncharacterized, role of miRNAs during early spermatogenesis. The goal of this study was to characterize on postnatal day 7, 10, and 14 the content and editing of murine testicular miRNAs, which predominantly arise from spermatogonia and spermatocytes, in contrast to prior descriptions of miRNAs in the adult mouse testis which largely reflects the content of spermatids. Previous studies have shown miRNAs to be abundant in the mouse testis by postnatal day 14; however, through Next Generation Sequencing of testes from a B6;129 background we found abundant earlier expression of miRNAs and describe shifts in the miRNA signature during this period. We detected robust expression of miRNAs encoded on the X chromosome in postnatal day 14 testes, consistent with prior studies showing their resistance to meiotic sex chromosome inactivation. Unexpectedly, we also found a similar positional enrichment for most miRNAs on chromosome 2 at postnatal day 14 and for those on chromosome 12 at postnatal day 7. We quantified in vivo developmental changes in three types of miRNA variation including 5′ heterogeneity, editing, and 3′ nucleotide addition. We identified eleven putative novel pubertal testis miRNAs whose developmental expression suggests a possible role in early male germ cell development. These studies provide a foundation for interpretation of miRNA changes associated with testicular pathology and identification of novel components of the miRNA editing machinery in the testis. Public Library of Science 2010-12-29 /pmc/articles/PMC3012074/ /pubmed/21206922 http://dx.doi.org/10.1371/journal.pone.0015317 Text en Buchold et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Buchold, Gregory M. Coarfa, Cristian Kim, Jong Milosavljevic, Aleksandar Gunaratne, Preethi H. Matzuk, Martin M. Analysis of MicroRNA Expression in the Prepubertal Testis |
title | Analysis of MicroRNA Expression in the Prepubertal Testis |
title_full | Analysis of MicroRNA Expression in the Prepubertal Testis |
title_fullStr | Analysis of MicroRNA Expression in the Prepubertal Testis |
title_full_unstemmed | Analysis of MicroRNA Expression in the Prepubertal Testis |
title_short | Analysis of MicroRNA Expression in the Prepubertal Testis |
title_sort | analysis of microrna expression in the prepubertal testis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012074/ https://www.ncbi.nlm.nih.gov/pubmed/21206922 http://dx.doi.org/10.1371/journal.pone.0015317 |
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