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The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings
BACKGROUND: The Hawaiian red algal flora is diverse, isolated, and well studied from a morphological and anatomical perspective, making it an excellent candidate for assessment using a combination of traditional taxonomic and molecular approaches. Acquiring and making these biodiversity data freely...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012605/ https://www.ncbi.nlm.nih.gov/pubmed/21092229 http://dx.doi.org/10.1186/1471-2229-10-258 |
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author | Sherwood, Alison R Kurihara, Akira Conklin, Kimberly Y Sauvage, Thomas Presting, Gernot G |
author_facet | Sherwood, Alison R Kurihara, Akira Conklin, Kimberly Y Sauvage, Thomas Presting, Gernot G |
author_sort | Sherwood, Alison R |
collection | PubMed |
description | BACKGROUND: The Hawaiian red algal flora is diverse, isolated, and well studied from a morphological and anatomical perspective, making it an excellent candidate for assessment using a combination of traditional taxonomic and molecular approaches. Acquiring and making these biodiversity data freely available in a timely manner ensures that other researchers can incorporate these baseline findings into phylogeographic studies of Hawaiian red algae or red algae found in other locations. RESULTS: A total of 1,946 accessions are represented in the collections from 305 different geographical locations in the Hawaiian archipelago. These accessions represent 24 orders, 49 families, 152 genera and 252 species/subspecific taxa of red algae. One order of red algae (the Rhodachlyales) was recognized in Hawaii for the first time and 196 new island distributional records were determined from the survey collections. One family and four genera are reported for the first time from Hawaii, and multiple species descriptions are in progress for newly discovered taxa. A total of 2,418 sequences were generated for Hawaiian red algae in the course of this study - 915 for the nuclear LSU marker, 864 for the plastidial UPA marker, and 639 for the mitochondrial COI marker. These baseline molecular data are presented as neighbor-joining trees to illustrate degrees of divergence within and among taxa. The LSU marker was typically most conserved, followed by UPA and COI. Phylogenetic analysis of a set of concatenated LSU, UPA and COI sequences recovered a tree that broadly resembled the current understanding of florideophyte red algal relationships, but bootstrap support was largely absent above the ordinal level. Phylogeographic trends are reported here for some common taxa within the Hawaiian Islands and include examples of those with, as well as without, intraspecific variation. CONCLUSIONS: The UPA and COI markers were determined to be the most useful of the three and are recommended for inclusion in future algal biodiversity surveys. Molecular data for the survey provide the most extensive assessment of Hawaiian red algal diversity and, in combination with the morphological/anatomical and distributional data collected as part of the project, provide a solid baseline data set for future studies of the flora. The data are freely available via the Hawaiian Algal Database (HADB), which was designed and constructed to accommodate the results of the project. We present the first DNA sequence reference collection for a tropical Pacific seaweed flora, whose value extends beyond Hawaii since many Hawaiian taxa are shared with other tropical areas. |
format | Text |
id | pubmed-3012605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30126052010-12-31 The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings Sherwood, Alison R Kurihara, Akira Conklin, Kimberly Y Sauvage, Thomas Presting, Gernot G BMC Plant Biol Research Article BACKGROUND: The Hawaiian red algal flora is diverse, isolated, and well studied from a morphological and anatomical perspective, making it an excellent candidate for assessment using a combination of traditional taxonomic and molecular approaches. Acquiring and making these biodiversity data freely available in a timely manner ensures that other researchers can incorporate these baseline findings into phylogeographic studies of Hawaiian red algae or red algae found in other locations. RESULTS: A total of 1,946 accessions are represented in the collections from 305 different geographical locations in the Hawaiian archipelago. These accessions represent 24 orders, 49 families, 152 genera and 252 species/subspecific taxa of red algae. One order of red algae (the Rhodachlyales) was recognized in Hawaii for the first time and 196 new island distributional records were determined from the survey collections. One family and four genera are reported for the first time from Hawaii, and multiple species descriptions are in progress for newly discovered taxa. A total of 2,418 sequences were generated for Hawaiian red algae in the course of this study - 915 for the nuclear LSU marker, 864 for the plastidial UPA marker, and 639 for the mitochondrial COI marker. These baseline molecular data are presented as neighbor-joining trees to illustrate degrees of divergence within and among taxa. The LSU marker was typically most conserved, followed by UPA and COI. Phylogenetic analysis of a set of concatenated LSU, UPA and COI sequences recovered a tree that broadly resembled the current understanding of florideophyte red algal relationships, but bootstrap support was largely absent above the ordinal level. Phylogeographic trends are reported here for some common taxa within the Hawaiian Islands and include examples of those with, as well as without, intraspecific variation. CONCLUSIONS: The UPA and COI markers were determined to be the most useful of the three and are recommended for inclusion in future algal biodiversity surveys. Molecular data for the survey provide the most extensive assessment of Hawaiian red algal diversity and, in combination with the morphological/anatomical and distributional data collected as part of the project, provide a solid baseline data set for future studies of the flora. The data are freely available via the Hawaiian Algal Database (HADB), which was designed and constructed to accommodate the results of the project. We present the first DNA sequence reference collection for a tropical Pacific seaweed flora, whose value extends beyond Hawaii since many Hawaiian taxa are shared with other tropical areas. BioMed Central 2010-11-22 /pmc/articles/PMC3012605/ /pubmed/21092229 http://dx.doi.org/10.1186/1471-2229-10-258 Text en Copyright ©2010 Sherwood et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sherwood, Alison R Kurihara, Akira Conklin, Kimberly Y Sauvage, Thomas Presting, Gernot G The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title | The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title_full | The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title_fullStr | The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title_full_unstemmed | The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title_short | The Hawaiian Rhodophyta Biodiversity Survey (2006-2010): a summary of principal findings |
title_sort | hawaiian rhodophyta biodiversity survey (2006-2010): a summary of principal findings |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012605/ https://www.ncbi.nlm.nih.gov/pubmed/21092229 http://dx.doi.org/10.1186/1471-2229-10-258 |
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