Perfluorooctanoic Acid for Shotgun Proteomics

Here, we describe the novel use of a volatile surfactant, perfluorooctanoic acid (PFOA), for shotgun proteomics. PFOA was found to solubilize membrane proteins as effectively as sodium dodecyl sulfate (SDS). PFOA concentrations up to 0.5% (w/v) did not significantly inhibit trypsin activity. The uni...

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Detalles Bibliográficos
Autores principales: Kadiyala, Chandra Sekhar Rao, Tomechko, Sara E., Miyagi, Masaru
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012695/
https://www.ncbi.nlm.nih.gov/pubmed/21209883
http://dx.doi.org/10.1371/journal.pone.0015332

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012695/
https://www.ncbi.nlm.nih.gov/pubmed/21209883
http://dx.doi.org/10.1371/journal.pone.0015332

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