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Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia

BACKGROUND: In vertebrate olfactory receptor neurons, sensory cilia transduce odor stimuli into changes in neuronal membrane potential. The voltage changes are primarily caused by the sequential openings of two types of channel: a cyclic-nucleotide-gated (CNG) cationic channel and a calcium-gated ch...

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Detalles Bibliográficos
Autores principales: French, Donald A., Badamdorj, Dorjsuren, Kleene, Steven J.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012700/
https://www.ncbi.nlm.nih.gov/pubmed/21209888
http://dx.doi.org/10.1371/journal.pone.0015676
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author French, Donald A.
Badamdorj, Dorjsuren
Kleene, Steven J.
author_facet French, Donald A.
Badamdorj, Dorjsuren
Kleene, Steven J.
author_sort French, Donald A.
collection PubMed
description BACKGROUND: In vertebrate olfactory receptor neurons, sensory cilia transduce odor stimuli into changes in neuronal membrane potential. The voltage changes are primarily caused by the sequential openings of two types of channel: a cyclic-nucleotide-gated (CNG) cationic channel and a calcium-gated chloride channel. In frog, the cilia are 25 to 200 µm in length, so the spatial distributions of the channels may be an important determinant of odor sensitivity. PRINCIPAL FINDINGS: To determine the spatial distribution of the chloride channels, we recorded from single cilia as calcium was allowed to diffuse down the length of the cilium and activate the channels. A computational model of this experiment allowed an estimate of the spatial distribution of the chloride channels. On average, the channels were concentrated in a narrow band centered at a distance of 29% of the ciliary length, measured from the base of the cilium. This matches the location of the CNG channels determined previously. This non-uniform distribution of transduction proteins is consistent with similar findings in other cilia. CONCLUSIONS: On average, the two types of olfactory transduction channel are concentrated in the same region of the cilium. This may contribute to the efficient detection of weak stimuli.
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spelling pubmed-30127002011-01-05 Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia French, Donald A. Badamdorj, Dorjsuren Kleene, Steven J. PLoS One Research Article BACKGROUND: In vertebrate olfactory receptor neurons, sensory cilia transduce odor stimuli into changes in neuronal membrane potential. The voltage changes are primarily caused by the sequential openings of two types of channel: a cyclic-nucleotide-gated (CNG) cationic channel and a calcium-gated chloride channel. In frog, the cilia are 25 to 200 µm in length, so the spatial distributions of the channels may be an important determinant of odor sensitivity. PRINCIPAL FINDINGS: To determine the spatial distribution of the chloride channels, we recorded from single cilia as calcium was allowed to diffuse down the length of the cilium and activate the channels. A computational model of this experiment allowed an estimate of the spatial distribution of the chloride channels. On average, the channels were concentrated in a narrow band centered at a distance of 29% of the ciliary length, measured from the base of the cilium. This matches the location of the CNG channels determined previously. This non-uniform distribution of transduction proteins is consistent with similar findings in other cilia. CONCLUSIONS: On average, the two types of olfactory transduction channel are concentrated in the same region of the cilium. This may contribute to the efficient detection of weak stimuli. Public Library of Science 2010-12-30 /pmc/articles/PMC3012700/ /pubmed/21209888 http://dx.doi.org/10.1371/journal.pone.0015676 Text en French, et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
French, Donald A.
Badamdorj, Dorjsuren
Kleene, Steven J.
Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title_full Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title_fullStr Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title_full_unstemmed Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title_short Spatial Distribution of Calcium-Gated Chloride Channels in Olfactory Cilia
title_sort spatial distribution of calcium-gated chloride channels in olfactory cilia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3012700/
https://www.ncbi.nlm.nih.gov/pubmed/21209888
http://dx.doi.org/10.1371/journal.pone.0015676
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