Cargando…

Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector

The planthopper Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae) is an important vector of maize viruses in tropical and subtropical areas. Planthoppers are biologically controlled with several species of entomopathogenic fungi that have been isolated from these insect pests of rice in Asia. Bea...

Descripción completa

Detalles Bibliográficos
Autores principales: Toledo, A.V., de Remes Lenicov, A.M.M., López Lastra, C.C.
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014746/
https://www.ncbi.nlm.nih.gov/pubmed/20578956
http://dx.doi.org/10.1673/031.010.3501
_version_ 1782195384986107904
author Toledo, A.V.
de Remes Lenicov, A.M.M.
López Lastra, C.C.
author_facet Toledo, A.V.
de Remes Lenicov, A.M.M.
López Lastra, C.C.
author_sort Toledo, A.V.
collection PubMed
description The planthopper Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae) is an important vector of maize viruses in tropical and subtropical areas. Planthoppers are biologically controlled with several species of entomopathogenic fungi that have been isolated from these insect pests of rice in Asia. Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) appear to be the most useful against planthoppers because of their ease of mass production, storage, virulence, and application. In the present study, adults of P. maidis infected with B. bassiana and M. anisopliae were observed under light and scanning electron microscopy to characterize morphologically the process of infection and the development of these fungi, prior to and after the death of the host. The hydrophobic conidia of both fungal species were able to attach to all body regions, with a preference for surfaces containing hairs. Few germinated conidia were observed on the insect's body surface at 24, 48, and 72 hr post-inoculation. On the cuticular surface of P. maidis treated with B. bassiana and M. anisopliae, bacillus-like bacteria were observed. These microorganisms could be interacting with fungal conidia, playing a role of antibiosis that will not allow the fungal pathogens to germinate and penetrate. In the colonization events observed in this study, the formation and multiplication of hyphal bodies by both fungal species inside the host's body was noted. The host's whole body was invaded by hyphae between five and six days post-inoculation, and body fat was the most affected tissue.
format Text
id pubmed-3014746
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher University of Wisconsin Library
record_format MEDLINE/PubMed
spelling pubmed-30147462012-02-09 Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector Toledo, A.V. de Remes Lenicov, A.M.M. López Lastra, C.C. J Insect Sci Article The planthopper Peregrinus maidis (Ashmead) (Hemiptera: Delphacidae) is an important vector of maize viruses in tropical and subtropical areas. Planthoppers are biologically controlled with several species of entomopathogenic fungi that have been isolated from these insect pests of rice in Asia. Beauveria bassiana (Balsamo-Crivelli) Vuillemin and Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae) appear to be the most useful against planthoppers because of their ease of mass production, storage, virulence, and application. In the present study, adults of P. maidis infected with B. bassiana and M. anisopliae were observed under light and scanning electron microscopy to characterize morphologically the process of infection and the development of these fungi, prior to and after the death of the host. The hydrophobic conidia of both fungal species were able to attach to all body regions, with a preference for surfaces containing hairs. Few germinated conidia were observed on the insect's body surface at 24, 48, and 72 hr post-inoculation. On the cuticular surface of P. maidis treated with B. bassiana and M. anisopliae, bacillus-like bacteria were observed. These microorganisms could be interacting with fungal conidia, playing a role of antibiosis that will not allow the fungal pathogens to germinate and penetrate. In the colonization events observed in this study, the formation and multiplication of hyphal bodies by both fungal species inside the host's body was noted. The host's whole body was invaded by hyphae between five and six days post-inoculation, and body fat was the most affected tissue. University of Wisconsin Library 2010-04-17 /pmc/articles/PMC3014746/ /pubmed/20578956 http://dx.doi.org/10.1673/031.010.3501 Text en © 2010 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Toledo, A.V.
de Remes Lenicov, A.M.M.
López Lastra, C.C.
Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title_full Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title_fullStr Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title_full_unstemmed Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title_short Histopathology Caused by the Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae, in the Adult Planthopper, Peregrinus maidis, a Maize Virus Vector
title_sort histopathology caused by the entomopathogenic fungi, beauveria bassiana and metarhizium anisopliae, in the adult planthopper, peregrinus maidis, a maize virus vector
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014746/
https://www.ncbi.nlm.nih.gov/pubmed/20578956
http://dx.doi.org/10.1673/031.010.3501
work_keys_str_mv AT toledoav histopathologycausedbytheentomopathogenicfungibeauveriabassianaandmetarhiziumanisopliaeintheadultplanthopperperegrinusmaidisamaizevirusvector
AT deremeslenicovamm histopathologycausedbytheentomopathogenicfungibeauveriabassianaandmetarhiziumanisopliaeintheadultplanthopperperegrinusmaidisamaizevirusvector
AT lopezlastracc histopathologycausedbytheentomopathogenicfungibeauveriabassianaandmetarhiziumanisopliaeintheadultplanthopperperegrinusmaidisamaizevirusvector