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Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene
A TaqMan-based real-time polymerase chain reaction (PCR) assay was devised for the detection of porcine parvovirus (PPV). Two primers and a TaqMan probe for the non-structural protein NS1 gene were designed. The detection limit was 1 × 10(2 )DNA copies/μL, and the assay was linear in the range of 1...
| Autores principales: | , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014914/ https://www.ncbi.nlm.nih.gov/pubmed/21126330 http://dx.doi.org/10.1186/1743-422X-7-353 |