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Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation
Over 50 years of biological research with bacteriophage T4 includes notable discoveries in post-transcriptional control, including the genetic code, mRNA, and tRNA; the very foundations of molecular biology. In this review we compile the past 10 - 15 year literature on RNA-protein interactions with...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014915/ https://www.ncbi.nlm.nih.gov/pubmed/21129205 http://dx.doi.org/10.1186/1743-422X-7-360 |
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author | Uzan, Marc Miller, Eric S |
author_facet | Uzan, Marc Miller, Eric S |
author_sort | Uzan, Marc |
collection | PubMed |
description | Over 50 years of biological research with bacteriophage T4 includes notable discoveries in post-transcriptional control, including the genetic code, mRNA, and tRNA; the very foundations of molecular biology. In this review we compile the past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages, with particular focus on advances in mRNA decay and processing, and on translational repression. Binding of T4 proteins RegB, RegA, gp32 and gp43 to their cognate target RNAs has been characterized. For several of these, further study is needed for an atomic-level perspective, where resolved structures of RNA-protein complexes are awaiting investigation. Other features of post-transcriptional control are also summarized. These include: RNA structure at translation initiation regions that either inhibit or promote translation initiation; programmed translational bypassing, where T4 orchestrates ribosome bypass of a 50 nucleotide mRNA sequence; phage exclusion systems that involve T4-mediated activation of a latent endoribonuclease (PrrC) and cofactor-assisted activation of EF-Tu proteolysis (Gol-Lit); and potentially important findings on ADP-ribosylation (by Alt and Mod enzymes) of ribosome-associated proteins that might broadly impact protein synthesis in the infected cell. Many of these problems can continue to be addressed with T4, whereas the growing database of T4-related phage genome sequences provides new resources and potentially new phage-host systems to extend the work into a broader biological, evolutionary context. |
format | Text |
id | pubmed-3014915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-30149152011-01-05 Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation Uzan, Marc Miller, Eric S Virol J Review Over 50 years of biological research with bacteriophage T4 includes notable discoveries in post-transcriptional control, including the genetic code, mRNA, and tRNA; the very foundations of molecular biology. In this review we compile the past 10 - 15 year literature on RNA-protein interactions with T4 and some of its related phages, with particular focus on advances in mRNA decay and processing, and on translational repression. Binding of T4 proteins RegB, RegA, gp32 and gp43 to their cognate target RNAs has been characterized. For several of these, further study is needed for an atomic-level perspective, where resolved structures of RNA-protein complexes are awaiting investigation. Other features of post-transcriptional control are also summarized. These include: RNA structure at translation initiation regions that either inhibit or promote translation initiation; programmed translational bypassing, where T4 orchestrates ribosome bypass of a 50 nucleotide mRNA sequence; phage exclusion systems that involve T4-mediated activation of a latent endoribonuclease (PrrC) and cofactor-assisted activation of EF-Tu proteolysis (Gol-Lit); and potentially important findings on ADP-ribosylation (by Alt and Mod enzymes) of ribosome-associated proteins that might broadly impact protein synthesis in the infected cell. Many of these problems can continue to be addressed with T4, whereas the growing database of T4-related phage genome sequences provides new resources and potentially new phage-host systems to extend the work into a broader biological, evolutionary context. BioMed Central 2010-12-03 /pmc/articles/PMC3014915/ /pubmed/21129205 http://dx.doi.org/10.1186/1743-422X-7-360 Text en Copyright ©2010 Uzan and Miller; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Uzan, Marc Miller, Eric S Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title | Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title_full | Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title_fullStr | Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title_full_unstemmed | Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title_short | Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation |
title_sort | post-transcriptional control by bacteriophage t4: mrna decay and inhibition of translation initiation |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3014915/ https://www.ncbi.nlm.nih.gov/pubmed/21129205 http://dx.doi.org/10.1186/1743-422X-7-360 |
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