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Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture

Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropo...

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Autores principales: Yamauchi, Yoshinori, Tsuruga, Eichi, Nakashima, Kazuki, Sawa, Yoshihiko, Ishikawa, Hiroyuki
Formato: Texto
Lenguaje:English
Publicado: Japan Society of Histochemistry and Cytochemistry 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3015050/
https://www.ncbi.nlm.nih.gov/pubmed/21245979
http://dx.doi.org/10.1267/ahc.10026
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author Yamauchi, Yoshinori
Tsuruga, Eichi
Nakashima, Kazuki
Sawa, Yoshihiko
Ishikawa, Hiroyuki
author_facet Yamauchi, Yoshinori
Tsuruga, Eichi
Nakashima, Kazuki
Sawa, Yoshihiko
Ishikawa, Hiroyuki
author_sort Yamauchi, Yoshinori
collection PubMed
description Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropoelastin deposition. Among the fibulin family, fibulin-2, -4 and -5 are capable of binding to tropoelastin and fibrillin-1. In the present study, we used the RNA interference (RNAi) technique to establish individual gene-specific knockdown of fibulin-2, -4 and -5 in elastin-producing cells (human gingival fibroblasts; HGF). We then examined the extracellular deposition of tropoelastin using immunofluorescence. RNAi-mediated down-regulation of fibulin-4 and -5 was responsible for the diminution of tropoelastin deposition. Suppression of fibulin-5 appeared to inhibit the formation of fibrillin-1 microfibrils, while that of fibulin-4 did not. Similar results to those for HGF were obtained with human dermal fibroblasts. These results suggest that fibulin-4 and -5 may be associated in different ways with the extracellular deposition of tropoelastin during elastic fiber formation in elastin-producing cells in culture.
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spelling pubmed-30150502011-01-18 Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture Yamauchi, Yoshinori Tsuruga, Eichi Nakashima, Kazuki Sawa, Yoshihiko Ishikawa, Hiroyuki Acta Histochem Cytochem Regular Article Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited. Fibrillin-1 is the major component of microfibrils. It is not clear whether elastic fiber-associated molecules, such as fibulins, contribute to tropoelastin deposition. Among the fibulin family, fibulin-2, -4 and -5 are capable of binding to tropoelastin and fibrillin-1. In the present study, we used the RNA interference (RNAi) technique to establish individual gene-specific knockdown of fibulin-2, -4 and -5 in elastin-producing cells (human gingival fibroblasts; HGF). We then examined the extracellular deposition of tropoelastin using immunofluorescence. RNAi-mediated down-regulation of fibulin-4 and -5 was responsible for the diminution of tropoelastin deposition. Suppression of fibulin-5 appeared to inhibit the formation of fibrillin-1 microfibrils, while that of fibulin-4 did not. Similar results to those for HGF were obtained with human dermal fibroblasts. These results suggest that fibulin-4 and -5 may be associated in different ways with the extracellular deposition of tropoelastin during elastic fiber formation in elastin-producing cells in culture. Japan Society of Histochemistry and Cytochemistry 2010-12-29 2010-11-20 /pmc/articles/PMC3015050/ /pubmed/21245979 http://dx.doi.org/10.1267/ahc.10026 Text en © 2010 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Article
Yamauchi, Yoshinori
Tsuruga, Eichi
Nakashima, Kazuki
Sawa, Yoshihiko
Ishikawa, Hiroyuki
Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title_full Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title_fullStr Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title_full_unstemmed Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title_short Fibulin-4 and -5, but not Fibulin-2, are Associated with Tropoelastin Deposition in Elastin-Producing Cell Culture
title_sort fibulin-4 and -5, but not fibulin-2, are associated with tropoelastin deposition in elastin-producing cell culture
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3015050/
https://www.ncbi.nlm.nih.gov/pubmed/21245979
http://dx.doi.org/10.1267/ahc.10026
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