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Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish

BACKGROUND: Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish t...

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Autores principales: Gallardo, Viviana E, Liang, Jin, Behra, Martine, Elkahloun, Abdel, Villablanca, Eduardo J, Russo, Vincenzo, Allende, Miguel L, Burgess, Shawn M
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016277/
https://www.ncbi.nlm.nih.gov/pubmed/21144052
http://dx.doi.org/10.1186/1471-213X-10-120
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author Gallardo, Viviana E
Liang, Jin
Behra, Martine
Elkahloun, Abdel
Villablanca, Eduardo J
Russo, Vincenzo
Allende, Miguel L
Burgess, Shawn M
author_facet Gallardo, Viviana E
Liang, Jin
Behra, Martine
Elkahloun, Abdel
Villablanca, Eduardo J
Russo, Vincenzo
Allende, Miguel L
Burgess, Shawn M
author_sort Gallardo, Viviana E
collection PubMed
description BACKGROUND: Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish to form this sensory system. As it migrates, the primordium deposits groups of cells called neuromasts, the specialized organs that contain the mechanosensory hair cells. Therefore the primordium provides both a model for studying collective directional cell migration and the differentiation of sensory cells from multipotent progenitor cells. RESULTS: Through the combined use of transgenic fish, Fluorescence Activated Cell Sorting and microarray analysis we identified a repertoire of key genes expressed in the migrating primordium and in differentiated neuromasts. We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway construction revealed functional relationships among the genes enriched in the migrating cell population. CONCLUSIONS: Our results demonstrate that this is a robust approach to globally analyze tissue-specific expression and we predict that many of the genes identified in this study will show critical functions in developmental events involving collective cell migration and possibly in pathological situations such as tumor metastasis.
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spelling pubmed-30162772011-01-06 Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish Gallardo, Viviana E Liang, Jin Behra, Martine Elkahloun, Abdel Villablanca, Eduardo J Russo, Vincenzo Allende, Miguel L Burgess, Shawn M BMC Dev Biol Research Article BACKGROUND: Development of the posterior lateral line (PLL) system in zebrafish involves cell migration, proliferation and differentiation of mechanosensory cells. The PLL forms when cranial placodal cells delaminate and become a coherent, migratory primordium that traverses the length of the fish to form this sensory system. As it migrates, the primordium deposits groups of cells called neuromasts, the specialized organs that contain the mechanosensory hair cells. Therefore the primordium provides both a model for studying collective directional cell migration and the differentiation of sensory cells from multipotent progenitor cells. RESULTS: Through the combined use of transgenic fish, Fluorescence Activated Cell Sorting and microarray analysis we identified a repertoire of key genes expressed in the migrating primordium and in differentiated neuromasts. We validated the specific expression in the primordium of a subset of the identified sequences by quantitative RT-PCR, and by in situ hybridization. We also show that interfering with the function of two genes, f11r and cd9b, defects in primordium migration are induced. Finally, pathway construction revealed functional relationships among the genes enriched in the migrating cell population. CONCLUSIONS: Our results demonstrate that this is a robust approach to globally analyze tissue-specific expression and we predict that many of the genes identified in this study will show critical functions in developmental events involving collective cell migration and possibly in pathological situations such as tumor metastasis. BioMed Central 2010-12-13 /pmc/articles/PMC3016277/ /pubmed/21144052 http://dx.doi.org/10.1186/1471-213X-10-120 Text en Copyright ©2010 Gallardo et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gallardo, Viviana E
Liang, Jin
Behra, Martine
Elkahloun, Abdel
Villablanca, Eduardo J
Russo, Vincenzo
Allende, Miguel L
Burgess, Shawn M
Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title_full Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title_fullStr Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title_full_unstemmed Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title_short Molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
title_sort molecular dissection of the migrating posterior lateral line primordium during early development in zebrafish
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016277/
https://www.ncbi.nlm.nih.gov/pubmed/21144052
http://dx.doi.org/10.1186/1471-213X-10-120
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