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A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues

BACKGROUND: RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies. Most of the current approaches for RNA quality assessment are not suitable for FFPE derived RNA. RESULTS: We have developed a sing...

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Detalles Bibliográficos
Autores principales: Takano, Elena A, Mikeska, Thomas, Dobrovic, Alexander, Byrne, David J, Fox, Stephen B
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016381/
https://www.ncbi.nlm.nih.gov/pubmed/21162754
http://dx.doi.org/10.1186/1472-6750-10-89