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A multiplex endpoint RT-PCR assay for quality assessment of RNA extracted from formalin-fixed paraffin-embedded tissues
BACKGROUND: RNA extracted from formalin-fixed paraffin-embedded (FFPE) samples is chemically modified and degraded, which compromises its use in gene expression studies. Most of the current approaches for RNA quality assessment are not suitable for FFPE derived RNA. RESULTS: We have developed a sing...
Autores principales: | Takano, Elena A, Mikeska, Thomas, Dobrovic, Alexander, Byrne, David J, Fox, Stephen B |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016381/ https://www.ncbi.nlm.nih.gov/pubmed/21162754 http://dx.doi.org/10.1186/1472-6750-10-89 |
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