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DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)

A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or ora...

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Autores principales: Turell, Michael J., Bunning, Michel, Ludwig, George V., Ortman, Brian, Chang, Jeff, Speaker, Tully, Spielman, Andrew, McLean, Robert, Komar, Nicholas, Gates, Robert, McNamara, Tracey, Creekmore, Terry, Farley, Linda, Mitchell, Carl J.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016768/
https://www.ncbi.nlm.nih.gov/pubmed/14519243
http://dx.doi.org/10.3201/eid0909.030025
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author Turell, Michael J.
Bunning, Michel
Ludwig, George V.
Ortman, Brian
Chang, Jeff
Speaker, Tully
Spielman, Andrew
McLean, Robert
Komar, Nicholas
Gates, Robert
McNamara, Tracey
Creekmore, Terry
Farley, Linda
Mitchell, Carl J.
author_facet Turell, Michael J.
Bunning, Michel
Ludwig, George V.
Ortman, Brian
Chang, Jeff
Speaker, Tully
Spielman, Andrew
McLean, Robert
Komar, Nicholas
Gates, Robert
McNamara, Tracey
Creekmore, Terry
Farley, Linda
Mitchell, Carl J.
author_sort Turell, Michael J.
collection PubMed
description A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or orally exposed to a placebo. After 6 weeks, crows were challenged subcutaneously with 10(5) plaque-forming units of WNV (New York 1999 strain). None of the placebo inoculated–placebo challenged birds died. While none of the 9 IM vaccine–inoculated birds died, 5 of 10 placebo-inoculated and 4 of 8 orally vaccinated birds died within 15 days after challenge. Peak viremia titers in birds with fatal WNV infection were substantially higher than those in birds that survived infection. Although oral administration of a single DNA vaccine dose failed to elicit an immune response or protect crows from WNV infection, IM administration of a single dose prevented death and was associated with reduced viremia.
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spelling pubmed-30167682011-01-20 DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) Turell, Michael J. Bunning, Michel Ludwig, George V. Ortman, Brian Chang, Jeff Speaker, Tully Spielman, Andrew McLean, Robert Komar, Nicholas Gates, Robert McNamara, Tracey Creekmore, Terry Farley, Linda Mitchell, Carl J. Emerg Infect Dis Research A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or orally exposed to a placebo. After 6 weeks, crows were challenged subcutaneously with 10(5) plaque-forming units of WNV (New York 1999 strain). None of the placebo inoculated–placebo challenged birds died. While none of the 9 IM vaccine–inoculated birds died, 5 of 10 placebo-inoculated and 4 of 8 orally vaccinated birds died within 15 days after challenge. Peak viremia titers in birds with fatal WNV infection were substantially higher than those in birds that survived infection. Although oral administration of a single DNA vaccine dose failed to elicit an immune response or protect crows from WNV infection, IM administration of a single dose prevented death and was associated with reduced viremia. Centers for Disease Control and Prevention 2003-09 /pmc/articles/PMC3016768/ /pubmed/14519243 http://dx.doi.org/10.3201/eid0909.030025 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Turell, Michael J.
Bunning, Michel
Ludwig, George V.
Ortman, Brian
Chang, Jeff
Speaker, Tully
Spielman, Andrew
McLean, Robert
Komar, Nicholas
Gates, Robert
McNamara, Tracey
Creekmore, Terry
Farley, Linda
Mitchell, Carl J.
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title_full DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title_fullStr DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title_full_unstemmed DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title_short DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
title_sort dna vaccine for west nile virus infection in fish crows (corvus ossifragus)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016768/
https://www.ncbi.nlm.nih.gov/pubmed/14519243
http://dx.doi.org/10.3201/eid0909.030025
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