Human Metapneumovirus Detection in Patients with Severe Acute Respiratory Syndrome

We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coron...

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Detalles Bibliográficos
Autores principales: Chan, Paul K.S., Tam, John S., Lam, Ching-Wan, Chan, Edward, Wu, Alan, Li, Chi-Kong, Buckley, Thomas A., Ng, King-Cheung, Joynt, Gavin M., Cheng, Frankie W.T., To, Ka-Fai, Lee, Nelson, Hui, David S.C., Cheung, Jo L.K., Chu, Ida, Liu, Esther, Chung, Sydney S.C., Sung, Joseph J.Y.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2003
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016779/
https://www.ncbi.nlm.nih.gov/pubmed/14519240
http://dx.doi.org/10.3201/eid0909.030304
Descripción
Sumario:We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.

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