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Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti

The presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the host-seeking behavior of mosquitoes. It is important to establish a method in...

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Detalles Bibliográficos
Autores principales: Sengul, Meryem S., Tu, Zhijian
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016889/
https://www.ncbi.nlm.nih.gov/pubmed/21062207
http://dx.doi.org/10.1673/031.010.14131
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author Sengul, Meryem S.
Tu, Zhijian
author_facet Sengul, Meryem S.
Tu, Zhijian
author_sort Sengul, Meryem S.
collection PubMed
description The presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the host-seeking behavior of mosquitoes. It is important to establish a method in which a loss-of-function test can be performed to determine the possible role of these genes in olfaction. In this study, a double subgenomic Sindbis virus expression system was used to reduce the expression of two Obp genes in Aedes aegypti L (Diptera: Culicidae), AaegObp1 and AaegObp2. Quantitative real-time PCR analysis showed predominant expression of both genes in the female antennae, the primary olfactory tissue of mosquitoes. Moreover, at 11 days post virus-inoculation, the mRNA levels of AaegObp1 and AaegObp2 were significantly reduced in olfactory tissues of recombinant virus-inoculated female mosquitoes compared to that of controls by approximately 8 and 100-fold, respectively. These data suggest that the double subgenomic Sindbis virus expression system can be efficiently used to knockdown Obp gene expression in olfactory tissues of mosquitoes. We discuss the potential for a systematic analysis of the molecular players involved in mosquito olfaction using this newly developed technique. Such analysis will provide an important step to interfere with the host-seeking behavior of mosquitoes to prevent the transmission of diseases.
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spelling pubmed-30168892012-02-09 Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti Sengul, Meryem S. Tu, Zhijian J Insect Sci Article The presence and expression of odorant-binding proteins (OBPs) in the olfactory organs suggest that they play an important role in mosquito olfaction. However, no direct evidence has been found for their involvement in the host-seeking behavior of mosquitoes. It is important to establish a method in which a loss-of-function test can be performed to determine the possible role of these genes in olfaction. In this study, a double subgenomic Sindbis virus expression system was used to reduce the expression of two Obp genes in Aedes aegypti L (Diptera: Culicidae), AaegObp1 and AaegObp2. Quantitative real-time PCR analysis showed predominant expression of both genes in the female antennae, the primary olfactory tissue of mosquitoes. Moreover, at 11 days post virus-inoculation, the mRNA levels of AaegObp1 and AaegObp2 were significantly reduced in olfactory tissues of recombinant virus-inoculated female mosquitoes compared to that of controls by approximately 8 and 100-fold, respectively. These data suggest that the double subgenomic Sindbis virus expression system can be efficiently used to knockdown Obp gene expression in olfactory tissues of mosquitoes. We discuss the potential for a systematic analysis of the molecular players involved in mosquito olfaction using this newly developed technique. Such analysis will provide an important step to interfere with the host-seeking behavior of mosquitoes to prevent the transmission of diseases. University of Wisconsin Library 2010-10-05 /pmc/articles/PMC3016889/ /pubmed/21062207 http://dx.doi.org/10.1673/031.010.14131 Text en © 2010 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Sengul, Meryem S.
Tu, Zhijian
Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title_full Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title_fullStr Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title_full_unstemmed Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title_short Expression Analysis and Knockdown of Two Antennal Odorant-Binding Protein Genes in Aedes aegypti
title_sort expression analysis and knockdown of two antennal odorant-binding protein genes in aedes aegypti
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016889/
https://www.ncbi.nlm.nih.gov/pubmed/21062207
http://dx.doi.org/10.1673/031.010.14131
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