Cargando…

The SUMO protease SENP6 is a direct regulator of PML nuclear bodies

Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be...

Descripción completa

Detalles Bibliográficos
Autores principales: Hattersley, Neil, Shen, Linnan, Jaffray, Ellis G., Hay, Ronald T.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016979/
https://www.ncbi.nlm.nih.gov/pubmed/21148299
http://dx.doi.org/10.1091/mbc.E10-06-0504
_version_ 1782195850941825024
author Hattersley, Neil
Shen, Linnan
Jaffray, Ellis G.
Hay, Ronald T.
author_facet Hattersley, Neil
Shen, Linnan
Jaffray, Ellis G.
Hay, Ronald T.
author_sort Hattersley, Neil
collection PubMed
description Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be specific for SUMO-2/3–modified substrates and shows preference for SUMO polymers. Here, we further investigate the substrate specificity of SENP6 and show that it is also capable of cleaving mixed chains of SUMO-1 and SUMO-2/3. Depletion of SENP6 results in accumulation of endogenous SUMO-2/3 and SUMO-1 conjugates, and immunofluorescence analysis shows accumulation of SUMO and PML in an increased number of PML NBs. Although SENP6 depletion drastically increases the size of PML NBs, the organizational structure of the body is not affected. Mutation of the catalytic cysteine of SENP6 results in its accumulation in PML NBs, and biochemical analysis indicates that SUMO-modified PML is a substrate of SENP6.
format Text
id pubmed-3016979
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-30169792011-03-16 The SUMO protease SENP6 is a direct regulator of PML nuclear bodies Hattersley, Neil Shen, Linnan Jaffray, Ellis G. Hay, Ronald T. Mol Biol Cell Articles Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be specific for SUMO-2/3–modified substrates and shows preference for SUMO polymers. Here, we further investigate the substrate specificity of SENP6 and show that it is also capable of cleaving mixed chains of SUMO-1 and SUMO-2/3. Depletion of SENP6 results in accumulation of endogenous SUMO-2/3 and SUMO-1 conjugates, and immunofluorescence analysis shows accumulation of SUMO and PML in an increased number of PML NBs. Although SENP6 depletion drastically increases the size of PML NBs, the organizational structure of the body is not affected. Mutation of the catalytic cysteine of SENP6 results in its accumulation in PML NBs, and biochemical analysis indicates that SUMO-modified PML is a substrate of SENP6. The American Society for Cell Biology 2011-01-01 /pmc/articles/PMC3016979/ /pubmed/21148299 http://dx.doi.org/10.1091/mbc.E10-06-0504 Text en © 2011 Hattersley et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Hattersley, Neil
Shen, Linnan
Jaffray, Ellis G.
Hay, Ronald T.
The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title_full The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title_fullStr The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title_full_unstemmed The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title_short The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
title_sort sumo protease senp6 is a direct regulator of pml nuclear bodies
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016979/
https://www.ncbi.nlm.nih.gov/pubmed/21148299
http://dx.doi.org/10.1091/mbc.E10-06-0504
work_keys_str_mv AT hattersleyneil thesumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT shenlinnan thesumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT jaffrayellisg thesumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT hayronaldt thesumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT hattersleyneil sumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT shenlinnan sumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT jaffrayellisg sumoproteasesenp6isadirectregulatorofpmlnuclearbodies
AT hayronaldt sumoproteasesenp6isadirectregulatorofpmlnuclearbodies