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The SUMO protease SENP6 is a direct regulator of PML nuclear bodies
Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The American Society for Cell Biology
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016979/ https://www.ncbi.nlm.nih.gov/pubmed/21148299 http://dx.doi.org/10.1091/mbc.E10-06-0504 |
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author | Hattersley, Neil Shen, Linnan Jaffray, Ellis G. Hay, Ronald T. |
author_facet | Hattersley, Neil Shen, Linnan Jaffray, Ellis G. Hay, Ronald T. |
author_sort | Hattersley, Neil |
collection | PubMed |
description | Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be specific for SUMO-2/3–modified substrates and shows preference for SUMO polymers. Here, we further investigate the substrate specificity of SENP6 and show that it is also capable of cleaving mixed chains of SUMO-1 and SUMO-2/3. Depletion of SENP6 results in accumulation of endogenous SUMO-2/3 and SUMO-1 conjugates, and immunofluorescence analysis shows accumulation of SUMO and PML in an increased number of PML NBs. Although SENP6 depletion drastically increases the size of PML NBs, the organizational structure of the body is not affected. Mutation of the catalytic cysteine of SENP6 results in its accumulation in PML NBs, and biochemical analysis indicates that SUMO-modified PML is a substrate of SENP6. |
format | Text |
id | pubmed-3016979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-30169792011-03-16 The SUMO protease SENP6 is a direct regulator of PML nuclear bodies Hattersley, Neil Shen, Linnan Jaffray, Ellis G. Hay, Ronald T. Mol Biol Cell Articles Promyelocytic leukemia protein (PML) is the core component of PML-nuclear bodies (PML NBs). The small ubiquitin-like modifier (SUMO) system (and, in particular, SUMOylation of PML) is a critical component in the formation and regulation of PML NBs. SUMO protease SENP6 has been shown previously to be specific for SUMO-2/3–modified substrates and shows preference for SUMO polymers. Here, we further investigate the substrate specificity of SENP6 and show that it is also capable of cleaving mixed chains of SUMO-1 and SUMO-2/3. Depletion of SENP6 results in accumulation of endogenous SUMO-2/3 and SUMO-1 conjugates, and immunofluorescence analysis shows accumulation of SUMO and PML in an increased number of PML NBs. Although SENP6 depletion drastically increases the size of PML NBs, the organizational structure of the body is not affected. Mutation of the catalytic cysteine of SENP6 results in its accumulation in PML NBs, and biochemical analysis indicates that SUMO-modified PML is a substrate of SENP6. The American Society for Cell Biology 2011-01-01 /pmc/articles/PMC3016979/ /pubmed/21148299 http://dx.doi.org/10.1091/mbc.E10-06-0504 Text en © 2011 Hattersley et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,“ “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology. |
spellingShingle | Articles Hattersley, Neil Shen, Linnan Jaffray, Ellis G. Hay, Ronald T. The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title | The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title_full | The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title_fullStr | The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title_full_unstemmed | The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title_short | The SUMO protease SENP6 is a direct regulator of PML nuclear bodies |
title_sort | sumo protease senp6 is a direct regulator of pml nuclear bodies |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016979/ https://www.ncbi.nlm.nih.gov/pubmed/21148299 http://dx.doi.org/10.1091/mbc.E10-06-0504 |
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