Why Does Insect RNA Look Degraded?

The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile di...

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Detalles Bibliográficos
Autores principales: Winnebeck, Eva C., Millar, Craig D., Warman, Guy R.
Formato: Texto
Lenguaje:English
Publicado: University of Wisconsin Library 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016993/
https://www.ncbi.nlm.nih.gov/pubmed/21067419
http://dx.doi.org/10.1673/031.010.14119
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author Winnebeck, Eva C.
Millar, Craig D.
Warman, Guy R.
author_facet Winnebeck, Eva C.
Millar, Craig D.
Warman, Guy R.
author_sort Winnebeck, Eva C.
collection PubMed
description The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous “hidden break.” Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation.
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spelling pubmed-30169932012-02-09 Why Does Insect RNA Look Degraded? Winnebeck, Eva C. Millar, Craig D. Warman, Guy R. J Insect Sci Article The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous “hidden break.” Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation. University of Wisconsin Library 2010-09-21 /pmc/articles/PMC3016993/ /pubmed/21067419 http://dx.doi.org/10.1673/031.010.14119 Text en © 2010 http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Article
Winnebeck, Eva C.
Millar, Craig D.
Warman, Guy R.
Why Does Insect RNA Look Degraded?
title Why Does Insect RNA Look Degraded?
title_full Why Does Insect RNA Look Degraded?
title_fullStr Why Does Insect RNA Look Degraded?
title_full_unstemmed Why Does Insect RNA Look Degraded?
title_short Why Does Insect RNA Look Degraded?
title_sort why does insect rna look degraded?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3016993/
https://www.ncbi.nlm.nih.gov/pubmed/21067419
http://dx.doi.org/10.1673/031.010.14119
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