Cargando…

Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction

BACKGROUND: Plant growth depends on both cell division and cell expansion. Plant hormones, including brassinosteroids (BRs), are central to the control of these two cellular processes. Despite clear evidence that BRs regulate cell elongation, their roles in cell division have remained elusive. RESUL...

Descripción completa

Detalles Bibliográficos
Autores principales: Cheon, Jinyeong, Park, So-Young, Schulz, Burkhard, Choe, Sunghwa
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017067/
https://www.ncbi.nlm.nih.gov/pubmed/21143877
http://dx.doi.org/10.1186/1471-2229-10-270
_version_ 1782195875761618944
author Cheon, Jinyeong
Park, So-Young
Schulz, Burkhard
Choe, Sunghwa
author_facet Cheon, Jinyeong
Park, So-Young
Schulz, Burkhard
Choe, Sunghwa
author_sort Cheon, Jinyeong
collection PubMed
description BACKGROUND: Plant growth depends on both cell division and cell expansion. Plant hormones, including brassinosteroids (BRs), are central to the control of these two cellular processes. Despite clear evidence that BRs regulate cell elongation, their roles in cell division have remained elusive. RESULTS: Here, we report results emphasizing the importance of BRs in cell division. An Arabidopsis BR biosynthetic mutant, dwarf7-1, displayed various characteristics attributable to slower cell division rates. We found that the DWARF4 gene which encodes for an enzyme catalyzing a rate-determining step in the BR biosynthetic pathways, is highly expressed in the actively dividing callus, suggesting that BR biosynthesis is necessary for dividing cells. Furthermore, dwf7-1 showed noticeably slower rates of callus growth and shoot induction relative to wild-type control. Flow cytometric analyses of the nuclei derived from either calli or intact roots revealed that the cell division index, which was represented as the ratio of cells at the G2/M vs. G1 phases, was smaller in dwf7-1 plants. Finally, we found that the expression levels of the genes involved in cell division and shoot induction, such as PROLIFERATING CELL NUCLEAR ANTIGEN2 (PCNA2) and ENHANCER OF SHOOT REGENERATION2 (ESR2), were also lower in dwf7-1 as compared with wild type. CONCLUSIONS: Taken together, results of callus induction, shoot regeneration, flow cytometry, and semi-quantitative RT-PCR analysis suggest that BRs play important roles in both cell division and cell differentiation in Arabidopsis.
format Text
id pubmed-3017067
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-30170672011-01-07 Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction Cheon, Jinyeong Park, So-Young Schulz, Burkhard Choe, Sunghwa BMC Plant Biol Research Article BACKGROUND: Plant growth depends on both cell division and cell expansion. Plant hormones, including brassinosteroids (BRs), are central to the control of these two cellular processes. Despite clear evidence that BRs regulate cell elongation, their roles in cell division have remained elusive. RESULTS: Here, we report results emphasizing the importance of BRs in cell division. An Arabidopsis BR biosynthetic mutant, dwarf7-1, displayed various characteristics attributable to slower cell division rates. We found that the DWARF4 gene which encodes for an enzyme catalyzing a rate-determining step in the BR biosynthetic pathways, is highly expressed in the actively dividing callus, suggesting that BR biosynthesis is necessary for dividing cells. Furthermore, dwf7-1 showed noticeably slower rates of callus growth and shoot induction relative to wild-type control. Flow cytometric analyses of the nuclei derived from either calli or intact roots revealed that the cell division index, which was represented as the ratio of cells at the G2/M vs. G1 phases, was smaller in dwf7-1 plants. Finally, we found that the expression levels of the genes involved in cell division and shoot induction, such as PROLIFERATING CELL NUCLEAR ANTIGEN2 (PCNA2) and ENHANCER OF SHOOT REGENERATION2 (ESR2), were also lower in dwf7-1 as compared with wild type. CONCLUSIONS: Taken together, results of callus induction, shoot regeneration, flow cytometry, and semi-quantitative RT-PCR analysis suggest that BRs play important roles in both cell division and cell differentiation in Arabidopsis. BioMed Central 2010-12-09 /pmc/articles/PMC3017067/ /pubmed/21143877 http://dx.doi.org/10.1186/1471-2229-10-270 Text en Copyright ©2010 Cheon et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cheon, Jinyeong
Park, So-Young
Schulz, Burkhard
Choe, Sunghwa
Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title_full Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title_fullStr Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title_full_unstemmed Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title_short Arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
title_sort arabidopsis brassinosteroid biosynthetic mutant dwarf7-1 exhibits slower rates of cell division and shoot induction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017067/
https://www.ncbi.nlm.nih.gov/pubmed/21143877
http://dx.doi.org/10.1186/1471-2229-10-270
work_keys_str_mv AT cheonjinyeong arabidopsisbrassinosteroidbiosyntheticmutantdwarf71exhibitsslowerratesofcelldivisionandshootinduction
AT parksoyoung arabidopsisbrassinosteroidbiosyntheticmutantdwarf71exhibitsslowerratesofcelldivisionandshootinduction
AT schulzburkhard arabidopsisbrassinosteroidbiosyntheticmutantdwarf71exhibitsslowerratesofcelldivisionandshootinduction
AT choesunghwa arabidopsisbrassinosteroidbiosyntheticmutantdwarf71exhibitsslowerratesofcelldivisionandshootinduction