Cargando…

Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout

BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Mingyou, Shen, Qian, Xu, Hongyan, Wong, Foong Mei, Cui, Jianzhou, Li, Zhendong, Hong, Ni, Wang, Li, Zhao, Haobin, Ma, Bo, Hong, Yunhan
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017096/
https://www.ncbi.nlm.nih.gov/pubmed/21253610
http://dx.doi.org/10.1371/journal.pone.0015910
_version_ 1782195883616501760
author Li, Mingyou
Shen, Qian
Xu, Hongyan
Wong, Foong Mei
Cui, Jianzhou
Li, Zhendong
Hong, Ni
Wang, Li
Zhao, Haobin
Ma, Bo
Hong, Yunhan
author_facet Li, Mingyou
Shen, Qian
Xu, Hongyan
Wong, Foong Mei
Cui, Jianzhou
Li, Zhendong
Hong, Ni
Wang, Li
Zhao, Haobin
Ma, Bo
Hong, Yunhan
author_sort Li, Mingyou
collection PubMed
description BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells.
format Text
id pubmed-3017096
institution National Center for Biotechnology Information
language English
publishDate 2011
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-30170962011-01-20 Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout Li, Mingyou Shen, Qian Xu, Hongyan Wong, Foong Mei Cui, Jianzhou Li, Zhendong Hong, Ni Wang, Li Zhao, Haobin Ma, Bo Hong, Yunhan PLoS One Research Article BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells. Public Library of Science 2011-01-06 /pmc/articles/PMC3017096/ /pubmed/21253610 http://dx.doi.org/10.1371/journal.pone.0015910 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Li, Mingyou
Shen, Qian
Xu, Hongyan
Wong, Foong Mei
Cui, Jianzhou
Li, Zhendong
Hong, Ni
Wang, Li
Zhao, Haobin
Ma, Bo
Hong, Yunhan
Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title_full Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title_fullStr Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title_full_unstemmed Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title_short Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
title_sort differential conservation and divergence of fertility genes boule and dazl in the rainbow trout
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017096/
https://www.ncbi.nlm.nih.gov/pubmed/21253610
http://dx.doi.org/10.1371/journal.pone.0015910
work_keys_str_mv AT limingyou differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT shenqian differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT xuhongyan differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT wongfoongmei differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT cuijianzhou differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT lizhendong differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT hongni differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT wangli differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT zhaohaobin differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT mabo differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout
AT hongyunhan differentialconservationanddivergenceoffertilitygenesbouleanddazlintherainbowtrout