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Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout
BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017096/ https://www.ncbi.nlm.nih.gov/pubmed/21253610 http://dx.doi.org/10.1371/journal.pone.0015910 |
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author | Li, Mingyou Shen, Qian Xu, Hongyan Wong, Foong Mei Cui, Jianzhou Li, Zhendong Hong, Ni Wang, Li Zhao, Haobin Ma, Bo Hong, Yunhan |
author_facet | Li, Mingyou Shen, Qian Xu, Hongyan Wong, Foong Mei Cui, Jianzhou Li, Zhendong Hong, Ni Wang, Li Zhao, Haobin Ma, Bo Hong, Yunhan |
author_sort | Li, Mingyou |
collection | PubMed |
description | BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells. |
format | Text |
id | pubmed-3017096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-30170962011-01-20 Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout Li, Mingyou Shen, Qian Xu, Hongyan Wong, Foong Mei Cui, Jianzhou Li, Zhendong Hong, Ni Wang, Li Zhao, Haobin Ma, Bo Hong, Yunhan PLoS One Research Article BACKGROUND: The genes boule and dazl are members of the DAZ (Deleted in Azoospermia) family encoding RNA binding proteins essential for germ cell development. Although dazl exhibits bisexual expression in mitotic and meiotic germ cells in diverse animals, boule shows unisexual meiotic expression in invertebrates and mammals but a bisexual mitotic and meiotic expression in medaka. How boule and dazl have evolved different expression patterns in diverse organisms has remained unknown. METHODOLOGY AND PRINCIPAL FINDINGS: Here we chose the fish rainbow trout (Oncorhynchus mykiss) as a second lower vertebrate model to investigate the expression of boule and dazl. By molecular cloning and sequence comparison, we identified cDNAs encoding the trout Boule and Dazl proteins, which have a conserved RNA-recognition motif and a maximal similarity to their homologs. By RT-PCR analysis, adult RNA expression of trout boule and dazl is restricted to the gonads of both sexes. By chromogenic and two-color fluorescence in situ hybridization, we revealed bisexual and germline-specific expression of boule and dazl. We found that dazl displays conserved expression throughout gametogenesis and concentrates in the Balbinani's body of early oocytes and the chromatoid body of sperm. Surprisingly, boule exhibits mitotic and meiotic expression in the male but meiosis-specific expression in the female. CONCLUSIONS: Our data underscores differential conservation and divergence of DAZ family genes during vertebrate evolution. We propose a model in which the diversity of boule expression in sex and stage specificity might have resulted from selective loss or gain of its expression in one sex and mitotic germ cells. Public Library of Science 2011-01-06 /pmc/articles/PMC3017096/ /pubmed/21253610 http://dx.doi.org/10.1371/journal.pone.0015910 Text en Li et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Li, Mingyou Shen, Qian Xu, Hongyan Wong, Foong Mei Cui, Jianzhou Li, Zhendong Hong, Ni Wang, Li Zhao, Haobin Ma, Bo Hong, Yunhan Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title | Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title_full | Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title_fullStr | Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title_full_unstemmed | Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title_short | Differential Conservation and Divergence of Fertility Genes boule and dazl in the Rainbow Trout |
title_sort | differential conservation and divergence of fertility genes boule and dazl in the rainbow trout |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017096/ https://www.ncbi.nlm.nih.gov/pubmed/21253610 http://dx.doi.org/10.1371/journal.pone.0015910 |
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