An assessment of histone-modification antibody quality

We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among sp...

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Detalles Bibliográficos
Autores principales: Egelhofer, Thea A., Minoda, Aki, Klugman, Sarit, Lee, Kyungjoon, Kolasinska-Zwierz, Paulina, Alekseyenko, Artyom A., Cheung, Ming-Sin, Day, Daniel S., Gadel, Sarah, Gorchakov, Andrey A., Gu, Tingting, Kharchenko, Peter V., Kuan, Samantha, Latorre, Isabel, Linder-Basso, Daniela, Luu, Ying, Ngo, Queminh, Perry, Marc, Rechtsteiner, Andreas, Riddle, Nicole C., Schwartz, Yuri B., Shanower, Gregory A., Vielle, Anne, Ahringer, Julie, Elgin, Sarah C. R., Kuroda, Mitzi I., Pirrotta, Vincenzo, Ren, Bing, Strome, Susan, Park, Peter J., Karpen, Gary, Hawkins, R. David, Lieb, Jason D.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017233/
https://www.ncbi.nlm.nih.gov/pubmed/21131980
http://dx.doi.org/10.1038/nsmb.1972
Descripción
Sumario:We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among specific antibodies, over 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use and provide a website for posting new test results.

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