An assessment of histone-modification antibody quality

We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among sp...

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Autores principales: Egelhofer, Thea A., Minoda, Aki, Klugman, Sarit, Lee, Kyungjoon, Kolasinska-Zwierz, Paulina, Alekseyenko, Artyom A., Cheung, Ming-Sin, Day, Daniel S., Gadel, Sarah, Gorchakov, Andrey A., Gu, Tingting, Kharchenko, Peter V., Kuan, Samantha, Latorre, Isabel, Linder-Basso, Daniela, Luu, Ying, Ngo, Queminh, Perry, Marc, Rechtsteiner, Andreas, Riddle, Nicole C., Schwartz, Yuri B., Shanower, Gregory A., Vielle, Anne, Ahringer, Julie, Elgin, Sarah C. R., Kuroda, Mitzi I., Pirrotta, Vincenzo, Ren, Bing, Strome, Susan, Park, Peter J., Karpen, Gary, Hawkins, R. David, Lieb, Jason D.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017233/
https://www.ncbi.nlm.nih.gov/pubmed/21131980
http://dx.doi.org/10.1038/nsmb.1972
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author Egelhofer, Thea A.
Minoda, Aki
Klugman, Sarit
Lee, Kyungjoon
Kolasinska-Zwierz, Paulina
Alekseyenko, Artyom A.
Cheung, Ming-Sin
Day, Daniel S.
Gadel, Sarah
Gorchakov, Andrey A.
Gu, Tingting
Kharchenko, Peter V.
Kuan, Samantha
Latorre, Isabel
Linder-Basso, Daniela
Luu, Ying
Ngo, Queminh
Perry, Marc
Rechtsteiner, Andreas
Riddle, Nicole C.
Schwartz, Yuri B.
Shanower, Gregory A.
Vielle, Anne
Ahringer, Julie
Elgin, Sarah C. R.
Kuroda, Mitzi I.
Pirrotta, Vincenzo
Ren, Bing
Strome, Susan
Park, Peter J.
Karpen, Gary
Hawkins, R. David
Lieb, Jason D.
author_facet Egelhofer, Thea A.
Minoda, Aki
Klugman, Sarit
Lee, Kyungjoon
Kolasinska-Zwierz, Paulina
Alekseyenko, Artyom A.
Cheung, Ming-Sin
Day, Daniel S.
Gadel, Sarah
Gorchakov, Andrey A.
Gu, Tingting
Kharchenko, Peter V.
Kuan, Samantha
Latorre, Isabel
Linder-Basso, Daniela
Luu, Ying
Ngo, Queminh
Perry, Marc
Rechtsteiner, Andreas
Riddle, Nicole C.
Schwartz, Yuri B.
Shanower, Gregory A.
Vielle, Anne
Ahringer, Julie
Elgin, Sarah C. R.
Kuroda, Mitzi I.
Pirrotta, Vincenzo
Ren, Bing
Strome, Susan
Park, Peter J.
Karpen, Gary
Hawkins, R. David
Lieb, Jason D.
author_sort Egelhofer, Thea A.
collection PubMed
description We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among specific antibodies, over 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use and provide a website for posting new test results.
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spelling pubmed-30172332011-07-01 An assessment of histone-modification antibody quality Egelhofer, Thea A. Minoda, Aki Klugman, Sarit Lee, Kyungjoon Kolasinska-Zwierz, Paulina Alekseyenko, Artyom A. Cheung, Ming-Sin Day, Daniel S. Gadel, Sarah Gorchakov, Andrey A. Gu, Tingting Kharchenko, Peter V. Kuan, Samantha Latorre, Isabel Linder-Basso, Daniela Luu, Ying Ngo, Queminh Perry, Marc Rechtsteiner, Andreas Riddle, Nicole C. Schwartz, Yuri B. Shanower, Gregory A. Vielle, Anne Ahringer, Julie Elgin, Sarah C. R. Kuroda, Mitzi I. Pirrotta, Vincenzo Ren, Bing Strome, Susan Park, Peter J. Karpen, Gary Hawkins, R. David Lieb, Jason D. Nat Struct Mol Biol Article We report testing of the specificity and utility of over 200 antibodies raised against 57 different histone modifications, in Drosophila melanogaster, Caenorhabditis elegans and human cells. While most antibodies performed well, over 25% failed specificity tests by dot blot or western blot. Among specific antibodies, over 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use and provide a website for posting new test results. 2010-12-05 2011-01 /pmc/articles/PMC3017233/ /pubmed/21131980 http://dx.doi.org/10.1038/nsmb.1972 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Egelhofer, Thea A.
Minoda, Aki
Klugman, Sarit
Lee, Kyungjoon
Kolasinska-Zwierz, Paulina
Alekseyenko, Artyom A.
Cheung, Ming-Sin
Day, Daniel S.
Gadel, Sarah
Gorchakov, Andrey A.
Gu, Tingting
Kharchenko, Peter V.
Kuan, Samantha
Latorre, Isabel
Linder-Basso, Daniela
Luu, Ying
Ngo, Queminh
Perry, Marc
Rechtsteiner, Andreas
Riddle, Nicole C.
Schwartz, Yuri B.
Shanower, Gregory A.
Vielle, Anne
Ahringer, Julie
Elgin, Sarah C. R.
Kuroda, Mitzi I.
Pirrotta, Vincenzo
Ren, Bing
Strome, Susan
Park, Peter J.
Karpen, Gary
Hawkins, R. David
Lieb, Jason D.
An assessment of histone-modification antibody quality
title An assessment of histone-modification antibody quality
title_full An assessment of histone-modification antibody quality
title_fullStr An assessment of histone-modification antibody quality
title_full_unstemmed An assessment of histone-modification antibody quality
title_short An assessment of histone-modification antibody quality
title_sort assessment of histone-modification antibody quality
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017233/
https://www.ncbi.nlm.nih.gov/pubmed/21131980
http://dx.doi.org/10.1038/nsmb.1972
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