A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time

The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in...

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Detalles Bibliográficos
Autores principales: Ranjan, Amit, Mallick, Birendra Nath
Formato: Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2010
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017360/
https://www.ncbi.nlm.nih.gov/pubmed/21228908
http://dx.doi.org/10.3389/fneur.2010.00157
Descripción
Sumario:The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.

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