A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time

The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in...

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Detalles Bibliográficos
Autores principales: Ranjan, Amit, Mallick, Birendra Nath
Formato: Texto
Lenguaje:English
Publicado: Frontiers Research Foundation 2010
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017360/
https://www.ncbi.nlm.nih.gov/pubmed/21228908
http://dx.doi.org/10.3389/fneur.2010.00157
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author Ranjan, Amit
Mallick, Birendra Nath
author_facet Ranjan, Amit
Mallick, Birendra Nath
author_sort Ranjan, Amit
collection PubMed
description The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines.
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spelling pubmed-30173602011-01-12 A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time Ranjan, Amit Mallick, Birendra Nath Front Neurol Neuroscience The two major limitations of Golgi–Cox method are that staining takes very long time and it is inconsistent. In this paper we describe a modification of the Golgi–Cox method, in which the tissue blocks were maintained at 37 ± 1°C during chromation for only 24 h and consistent staining of neurons in rat brain sections were observed. The method is simple, reproducible, rapid, inexpensive, and provides uniform staining with very good resolution of neuronal soma, dendrites as well as spines. Frontiers Research Foundation 2010-12-28 /pmc/articles/PMC3017360/ /pubmed/21228908 http://dx.doi.org/10.3389/fneur.2010.00157 Text en Copyright © 2010 Ranjan and Mallick. http://www.frontiersin.org/licenseagreement This is an open-access article subject to an exclusive license agreement between the authors and the Frontiers Research Foundation, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are credited.
spellingShingle Neuroscience
Ranjan, Amit
Mallick, Birendra Nath
A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title_full A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title_fullStr A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title_full_unstemmed A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title_short A Modified Method for Consistent and Reliable Golgi–Cox Staining in Significantly Reduced Time
title_sort modified method for consistent and reliable golgi–cox staining in significantly reduced time
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017360/
https://www.ncbi.nlm.nih.gov/pubmed/21228908
http://dx.doi.org/10.3389/fneur.2010.00157
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