High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons

The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary el...

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Detalles Bibliográficos
Autores principales: Deshmane, Satish L., Mukerjee, Ruma, Fan, Shongshan, Sawaya, Bassel E.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017553/
https://www.ncbi.nlm.nih.gov/pubmed/21249135
http://dx.doi.org/10.1371/journal.pone.0016148
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author Deshmane, Satish L.
Mukerjee, Ruma
Fan, Shongshan
Sawaya, Bassel E.
author_facet Deshmane, Satish L.
Mukerjee, Ruma
Fan, Shongshan
Sawaya, Bassel E.
author_sort Deshmane, Satish L.
collection PubMed
description The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders.
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spelling pubmed-30175532011-01-19 High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons Deshmane, Satish L. Mukerjee, Ruma Fan, Shongshan Sawaya, Bassel E. PLoS One Research Article The HIV-1 protein, Tat has been implicated in AIDS pathogenesis however, the amount of circulating Tat is believed to be very low and its quantification has been difficult. We performed the quantification of Tat released from infected cells and taken up by neurons using high performance capillary electrophoresis. This is the first report to successfully measure the amount of Tat in neurons and places Tat as a key player involved in HIV-associated neurocognitive disorders. Public Library of Science 2011-01-07 /pmc/articles/PMC3017553/ /pubmed/21249135 http://dx.doi.org/10.1371/journal.pone.0016148 Text en Deshmane et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Deshmane, Satish L.
Mukerjee, Ruma
Fan, Shongshan
Sawaya, Bassel E.
High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title_full High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title_fullStr High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title_full_unstemmed High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title_short High-Performance Capillary Electrophoresis for Determining HIV-1 Tat Protein in Neurons
title_sort high-performance capillary electrophoresis for determining hiv-1 tat protein in neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017553/
https://www.ncbi.nlm.nih.gov/pubmed/21249135
http://dx.doi.org/10.1371/journal.pone.0016148
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