Natural and engineered nicking endonucleases—from cleavage mechanism to engineering of strand-specificity

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Restriction endonucleases (REases) are highly specific DNA scissors that have facilitated the development of modern molecular biology. Intensive studies of double strand (ds) cleavage activity of Type IIP REases, which recognize 4–8 bp palindromic sequences, have revealed a variety of mechanisms of...

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Detalles Bibliográficos
Autores principales: Chan, Siu-Hong, Stoddard, Barry L., Xu, Shuang-yong
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2011
Materias:
Survey and Summary
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3017599/
https://www.ncbi.nlm.nih.gov/pubmed/20805246
http://dx.doi.org/10.1093/nar/gkq742

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